From: Versatile biocatalysis of fungal cytochrome P450 monooxygenases
CYP | CYP source | Expression host | Expression level | Substrate | CPR source | Kinetic parameters | Notes | Ref |
---|---|---|---|---|---|---|---|---|
CYP53A1 | A. niger | A. niger | 160 pmol/mg of protein (Microsomal fraction) | Benzoic acid | A. niger | k cat ; 270 ± 30 nmol min−1 nmol P450−1, K m; 0.083 ± 0.009 mM | Para-hydroxylation | [94] |
CYP65A2 | P. chrysosporium | S. cerevisiae | 1 nmol/L | Naringenin | S. cerevisiae | k cat ; 0.29 ± 0.02 min−1, K m; 391 ± 27 μM | 3′-Hydroxylation | [48] |
CYP50C | P. chrysosporium | S. cerevisiae | 40 pmol/mg of protein (Microsomal fraction) | Flavone | S. cerevisiae | k cat ; 0.52 ± 0.03 min−1, n; 2.2 ± 0.1, K S; 109 ± 6 µM | 3′-Hydroxylation, Hill equation was used for kinetic parameters | [49] |
CYP142C | P. chrysosporium | S. cerevisiae | 119 pmol/mg of protein (Microsomal fraction) | Flavone | S. cerevisiae | k cat ; 0.019 ± 0.002 min−1, n; 2.0 ± 0.4, K S; 152 ± 30 µM | 3′-Hydroxylation, Hill equation was used for kinetic parameters | [49] |
CYP52M1 | S. bombicola | S. cerevisiae | NA | Oleic acid | Arabidopsis thaliana | k cat ; 535 ± 60 pmol min−1 mg P450−1, K m; 40 ± 2 µM | ω-Hydroxylation | [44] |
PcCYP1f | P. chrysosporium | P. pastoris | NA | Benzoic acid | P. chrysosporium | k cat ; 0.013 μmol min−1μmol P450−1, K m; 185 µM | Para-hydroxylation | [39] |
CYP5150A2 | P. chrysosporium | E. coli | 200–300 nmol/L | 4-Propylbenzoic acid | P. chrysosporium | Initial velocity (μmol/min/μmol of P450); 0.3 ± 0.1 with CPR, 0.6 ± 0.1 with CPR, Cyt b5 and 10.6 ± 0.3 with CB5R, Cyt b5 | Formation of 4-(2-hydroxypropyl)benzoic acid, ∆ 13 form of CYP5150A2 coexpressed with GroEL/ES. CB5R/Cyt b5 act as electron partner in the absence of CPR | [31] |
MgCYP51 | M. graminicola | E. coli | NA | Eburicol | M. graminicola | k cat ; 0.13 min−1, K m; 33 µM | 14α- demethylation, Sterol selective and Temperature sensitive enzyme | [33] |
CYP53A15 | C. lunatus | E. coli | 800–900 nmol/L | Benzoic acid | Mammal | k cat ; 1.4 ± 0.5 min−1, K m; 1.4 ± 0.2 mM | para-hydroxylation | [37] |
CYP52A21 | C. albicans | E. coli | 60 nmol/L | Dodecanoic acid | Rat | k cat ; 33 ± 1 min−1, K m; 57 ± 2 µM | ω-Hydroxylation | [74] |
CYP630B18 | G. clavigera | E. coli | 3.1 μmol/L | Oleic acid | G. clavigera | NA | ω-Hydroxylation | [47] |
304 CYP isoforms | P. chrysosporium and P. placenta | E. coli | 43–1255 nmol/L | NA | – | NA | 27 CYPs were expressed in active form with/without NTD modifications among 304 CYPs | [75] |