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Table 2 Representative examples of Fungal CYP expression and their kinetic parameters

From: Versatile biocatalysis of fungal cytochrome P450 monooxygenases

CYP

CYP source

Expression host

Expression level

Substrate

CPR source

Kinetic parameters

Notes

Ref

CYP53A1

A. niger

A. niger

160 pmol/mg of protein (Microsomal fraction)

Benzoic acid

A. niger

k cat ; 270 ± 30 nmol min−1 nmol P450−1,

K m; 0.083 ± 0.009 mM

Para-hydroxylation

[94]

CYP65A2

P. chrysosporium

S. cerevisiae

1 nmol/L

Naringenin

S. cerevisiae

k cat ; 0.29 ± 0.02 min−1,

K m; 391 ± 27 μM

3′-Hydroxylation

[48]

CYP50C

P. chrysosporium

S. cerevisiae

40 pmol/mg of protein (Microsomal fraction)

Flavone

S. cerevisiae

k cat ; 0.52 ± 0.03 min−1,

n; 2.2 ± 0.1,

K S; 109 ± 6 µM

3′-Hydroxylation,

Hill equation was used for kinetic parameters

[49]

CYP142C

P. chrysosporium

S. cerevisiae

119 pmol/mg of protein (Microsomal fraction)

Flavone

S. cerevisiae

k cat ; 0.019 ± 0.002 min−1,

n; 2.0 ± 0.4,

K S; 152 ± 30 µM

3′-Hydroxylation,

Hill equation was used for kinetic parameters

[49]

CYP52M1

S. bombicola

S. cerevisiae

NA

Oleic acid

Arabidopsis thaliana

k cat ; 535 ± 60 pmol min−1 mg P450−1,

K m; 40 ± 2 µM

ω-Hydroxylation

[44]

PcCYP1f

P. chrysosporium

P. pastoris

NA

Benzoic acid

P. chrysosporium

k cat ; 0.013 μmol min−1μmol P450−1,

K m; 185 µM

Para-hydroxylation

[39]

CYP5150A2

P. chrysosporium

E. coli

200–300 nmol/L

4-Propylbenzoic acid

P. chrysosporium

Initial velocity (μmol/min/μmol of P450); 0.3 ± 0.1 with CPR, 0.6 ± 0.1 with CPR, Cyt b5 and 10.6 ± 0.3 with CB5R, Cyt b5

Formation of 4-(2-hydroxypropyl)benzoic acid,

∆ 13 form of CYP5150A2 coexpressed with GroEL/ES.

CB5R/Cyt b5 act as electron partner in the absence of CPR

[31]

MgCYP51

M. graminicola

E. coli

NA

Eburicol

M. graminicola

k cat ; 0.13 min−1,

K m; 33 µM

14α- demethylation,

Sterol selective and Temperature sensitive enzyme

[33]

CYP53A15

C. lunatus

E. coli

800–900 nmol/L

Benzoic acid

Mammal

k cat ; 1.4 ± 0.5 min−1,

K m; 1.4 ± 0.2 mM

para-hydroxylation

[37]

CYP52A21

C. albicans

E. coli

60 nmol/L

Dodecanoic acid

Rat

k cat ; 33 ± 1 min−1,

K m; 57 ± 2 µM

ω-Hydroxylation

[74]

CYP630B18

G. clavigera

E. coli

3.1 μmol/L

Oleic acid

G. clavigera

NA

ω-Hydroxylation

[47]

304 CYP isoforms

P. chrysosporium and P. placenta

E. coli

43–1255 nmol/L

NA

–

NA

27 CYPs were expressed in active form with/without NTD modifications among 304 CYPs

[75]

  1. NA information not available