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Table 2 Representative examples of Fungal CYP expression and their kinetic parameters

From: Versatile biocatalysis of fungal cytochrome P450 monooxygenases

CYP CYP source Expression host Expression level Substrate CPR source Kinetic parameters Notes Ref
CYP53A1 A. niger A. niger 160 pmol/mg of protein (Microsomal fraction) Benzoic acid A. niger k cat ; 270 ± 30 nmol min−1 nmol P450−1,
K m; 0.083 ± 0.009 mM
Para-hydroxylation [94]
CYP65A2 P. chrysosporium S. cerevisiae 1 nmol/L Naringenin S. cerevisiae k cat ; 0.29 ± 0.02 min−1,
K m; 391 ± 27 μM
3′-Hydroxylation [48]
CYP50C P. chrysosporium S. cerevisiae 40 pmol/mg of protein (Microsomal fraction) Flavone S. cerevisiae k cat ; 0.52 ± 0.03 min−1,
n; 2.2 ± 0.1,
K S; 109 ± 6 µM
3′-Hydroxylation,
Hill equation was used for kinetic parameters
[49]
CYP142C P. chrysosporium S. cerevisiae 119 pmol/mg of protein (Microsomal fraction) Flavone S. cerevisiae k cat ; 0.019 ± 0.002 min−1,
n; 2.0 ± 0.4,
K S; 152 ± 30 µM
3′-Hydroxylation,
Hill equation was used for kinetic parameters
[49]
CYP52M1 S. bombicola S. cerevisiae NA Oleic acid Arabidopsis thaliana k cat ; 535 ± 60 pmol min−1 mg P450−1,
K m; 40 ± 2 µM
ω-Hydroxylation [44]
PcCYP1f P. chrysosporium P. pastoris NA Benzoic acid P. chrysosporium k cat ; 0.013 μmol min−1μmol P450−1,
K m; 185 µM
Para-hydroxylation [39]
CYP5150A2 P. chrysosporium E. coli 200–300 nmol/L 4-Propylbenzoic acid P. chrysosporium Initial velocity (μmol/min/μmol of P450); 0.3 ± 0.1 with CPR, 0.6 ± 0.1 with CPR, Cyt b5 and 10.6 ± 0.3 with CB5R, Cyt b5 Formation of 4-(2-hydroxypropyl)benzoic acid,
∆ 13 form of CYP5150A2 coexpressed with GroEL/ES.
CB5R/Cyt b5 act as electron partner in the absence of CPR
[31]
MgCYP51 M. graminicola E. coli NA Eburicol M. graminicola k cat ; 0.13 min−1,
K m; 33 µM
14α- demethylation,
Sterol selective and Temperature sensitive enzyme
[33]
CYP53A15 C. lunatus E. coli 800–900 nmol/L Benzoic acid Mammal k cat ; 1.4 ± 0.5 min−1,
K m; 1.4 ± 0.2 mM
para-hydroxylation [37]
CYP52A21 C. albicans E. coli 60 nmol/L Dodecanoic acid Rat k cat ; 33 ± 1 min−1,
K m; 57 ± 2 µM
ω-Hydroxylation [74]
CYP630B18 G. clavigera E. coli 3.1 μmol/L Oleic acid G. clavigera NA ω-Hydroxylation [47]
304 CYP isoforms P. chrysosporium and P. placenta E. coli 43–1255 nmol/L NA NA 27 CYPs were expressed in active form with/without NTD modifications among 304 CYPs [75]
  1. NA information not available