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Table 1 Production of isotope-enriched r27RCL in P. pastoris and E. coli

From: Efficient production of 2H, 13C, 15N-enriched industrial enzyme Rhizopus chinensis lipase with native disulfide bonds

Expression systems

Expressed proteins

Expression level (mg/l)

Enrichment efficiency

P. pastoris

13C, 15N-enriched ppRCL

25.6 ± 3.1

83.6 ± 3.2 %

E. coli

13C, 15N-enriched ecRCL

15.3 ± 2.5

99.2 ± 0.2 %