Fig. 2From: Efficient production of 2H, 13C, 15N-enriched industrial enzyme Rhizopus chinensis lipase with native disulfide bondsExpression and purification of r27RCL by P. pastoris and E. coli. 1 soluble protein, 2 r27RCL(His)6 after HisTrap column, 3 soluble protein, 4 MBP-proRCL after one-step HisTrap-size-exclusion chromatography, 5 after Kex2 cleavage, 6 r27RCL after HisTrap columnBack to article page