Primer | Sequence (5′–3′) | Usage |
---|---|---|
Trcbh1pF | TGAGCGCGCGTAATACGACTCACTATAGGGCGAATTGATATCTAGAGTTGTGAAGTCGG | Cloning of cbh1 promoter |
Trcbh1Pr | GCACAATACGACTCCGGCGCTGGCCGATGCGCAGTCCGCGGTTGACTATT | Cloning of cbh1 promoter |
TrCBH1F | CCCAATAGTCAACCGCGGACTGCGCATCATGTATCGGAAGTTGGCCGTCATCTCGGCC | Cloning of cbh1 structural gene |
TrCBH1R | CACCAGAACCGTAGCGCTTGTCGCGCTCGAGCAGGCACTGAGAGTAGTAAGGGTTCAGG | Cloning of cbh1 structural gene |
NfBgl3AF | TTACTACTCTCAGTGCCTGCTCGAGCGCGACAAGCGCTACGGTTCTGGTGGCAGCAACTGGGATC | Cloning of NfBgl3A gene |
NfBgl3AR | TACGGGCTCACCAAGAATCTACCGGTGCGTCAGGCTTTCGCCACGGAGCTTCACCATCCGC GACGGACCCTGAAAGAG | Cloning of NfBgl3A gene |
Trcbh1tF | ACCACCACCACCACCACTAACTCGAGAGCTCCGTGGCGAAAGCCTGACGCACCGG | Cloning of cbh1 terminator |
Trcbh1tR | CCACCGCGGTGGCGGCCGCTCTAGAACTAGTGGATCCCCCGGGCTGCAGGGCGGCCGCAACA CTTCGGTGGAGGTGTCGAGTACG | Cloning of cbh1 terminator |
Yz-Trchb1F | GTTCGGACCCATTGGCAGCACCGG | Screening of positive transformants |
Yz-NfBgl3AR | AAGC GGATACCTAGCGGCGAGTCCTG | Screening of positive transformants |
q-NfBgl3AF | AATGGGCGCTGAGGCGAAGGGAC | qRT-PCR |
q-NfBgl3AR | TGAGAGCGGTGGTATCCACG | qRT-PCR |
q-tef1α-F | ACCAAGGCTGGCAAGTTC | qRT-PCR |
q-tef1α-R | GACACCAGTCTCGATACG | qRT-PCR |