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Table 1 Primers used in this study

From: Revisiting overexpression of a heterologous β-glucosidase in Trichoderma reesei: fusion expression of the Neosartorya fischeri Bgl3A to cbh1 enhances the overall as well as individual cellulase activities

Primer

Sequence (5′–3′)

Usage

Trcbh1pF

TGAGCGCGCGTAATACGACTCACTATAGGGCGAATTGATATCTAGAGTTGTGAAGTCGG

Cloning of cbh1 promoter

Trcbh1Pr

GCACAATACGACTCCGGCGCTGGCCGATGCGCAGTCCGCGGTTGACTATT

Cloning of cbh1 promoter

TrCBH1F

CCCAATAGTCAACCGCGGACTGCGCATCATGTATCGGAAGTTGGCCGTCATCTCGGCC

Cloning of cbh1 structural gene

TrCBH1R

CACCAGAACCGTAGCGCTTGTCGCGCTCGAGCAGGCACTGAGAGTAGTAAGGGTTCAGG

Cloning of cbh1 structural gene

NfBgl3AF

TTACTACTCTCAGTGCCTGCTCGAGCGCGACAAGCGCTACGGTTCTGGTGGCAGCAACTGGGATC

Cloning of NfBgl3A gene

NfBgl3AR

TACGGGCTCACCAAGAATCTACCGGTGCGTCAGGCTTTCGCCACGGAGCTTCACCATCCGC

GACGGACCCTGAAAGAG

Cloning of NfBgl3A gene

Trcbh1tF

ACCACCACCACCACCACTAACTCGAGAGCTCCGTGGCGAAAGCCTGACGCACCGG

Cloning of cbh1 terminator

Trcbh1tR

CCACCGCGGTGGCGGCCGCTCTAGAACTAGTGGATCCCCCGGGCTGCAGGGCGGCCGCAACA CTTCGGTGGAGGTGTCGAGTACG

Cloning of cbh1 terminator

Yz-Trchb1F

GTTCGGACCCATTGGCAGCACCGG

Screening of positive transformants

Yz-NfBgl3AR

AAGC GGATACCTAGCGGCGAGTCCTG

Screening of positive transformants

q-NfBgl3AF

AATGGGCGCTGAGGCGAAGGGAC

qRT-PCR

q-NfBgl3AR

TGAGAGCGGTGGTATCCACG

qRT-PCR

q-tef1α-F

ACCAAGGCTGGCAAGTTC

qRT-PCR

q-tef1α-R

GACACCAGTCTCGATACG

qRT-PCR