Fig. 5

Biochemical characterization of NfBgl3A expressed in T. reesei. a Effect of pH on β-glucosidase activity. The enzyme assays were performed at 80 °C for 10 min. b pH stability. The crude NfBgl3A was pre-incubated without substrate at 37 °C for 1 h and then subjected to assay of the residual activity under standard conditions (pH 5.0, 80 °C, 10 min). c Effect of temperature on NfBgl3A activity. The assays were carried out at pH 5.0 for 10 min. d Thermostability. The crude NfBgl3A was pre-incubated in the McIlvaine buffer (pH 5.0) at 70, 75, or 80 °C and the residual β-glucosidase activities were measured