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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Revisiting overexpression of a heterologous β-glucosidase in Trichoderma reesei: fusion expression of the Neosartorya fischeri Bgl3A to cbh1 enhances the overall as well as individual cellulase activities

Fig. 3

Transformation and expression of NfBgl3A in T. reesei. a Determination of NfBgl3A integration in the genome of T. reesei transformants by PCR using the primers Yz-Trchb1F/Yz-NfBgl3AR. The expected size is 445 bp, corresponding to a fragment spanning the joint region of cbh1 structural gene and NfBgl3A. Lane M DNA molecular mass marker; lane T1–T9, the transformants of T1–T9. b The β-glucosidase activities in the fermentation broth of TU-6 and six transformants

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Microbial Cell Factories

ISSN: 1475-2859

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