Transformation and expression of NfBgl3A in T. reesei.
a Determination of NfBgl3A integration in the genome of T. reesei transformants by PCR using the primers Yz-Trchb1F/Yz-NfBgl3AR. The expected size is 445 bp, corresponding to a fragment spanning the joint region of cbh1 structural gene and NfBgl3A. Lane M DNA molecular mass marker; lane T1–T9, the transformants of T1–T9. b The β-glucosidase activities in the fermentation broth of TU-6 and six transformants