Fig. 2From: Revisiting overexpression of a heterologous β-glucosidase in Trichoderma reesei: fusion expression of the Neosartorya fischeri Bgl3A to cbh1 enhances the overall as well as individual cellulase activitiesConstruction of pRS-NfBgl3A using DNA assembler. a Schematic diagram showing one-step assembly of pRS-NfBgl3A. b Restriction digestion analysis of pRS-NfBgl3A by NotI. Lane M DNA molecular weight marker; lane 1 KpnI-linearized pRS424; lane 2 undigested pRS-NfBgl3A; lane 3 NotI-digested pRS-NfBgl3A Back to article page