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Fig. 1 | Microbial Cell Factories

Fig. 1

From: Revisiting overexpression of a heterologous β-glucosidase in Trichoderma reesei: fusion expression of the Neosartorya fischeri Bgl3A to cbh1 enhances the overall as well as individual cellulase activities

Fig. 1

Supplementing with the P. pastoris-expressed NfBgl3A into the T. reesei cellulase enhanced release of glucose from crystalline cellulose. NfBgl3A was expressed in P. pastoris, purified, and added to TU-6 cellulase with increasing concentrations (0, 20, 25, 33, and 50 %, w/w). The enzymes were reacted with 20 mg/mL Avicel at 50 °C for 24 h. The released glucose and cellooligosaccharides were analyzed using HPAEC-PAD. C2-C6 represent cellobiose to cellohexaose, respectively

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Microbial Cell Factories

ISSN: 1475-2859

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