Fig. 2

Expression of PtME in C. pyrenoidosa. a Single PCR analysis. Lane 1–4 transformed algae; 5–8: wild type. a 1.68-kb band was detected in lanes 1–4 (transformed lines); while no such band was detected in lanes 5–8 (wild type). b Expression of PtME transcripts measured by qPCR; β-actin was used as internal reference gene. Significant difference between control and treatment groups is indicated at P < 0.05 (*) or P < 0.01 (**) level. Each value represents mean ± SD (n = 3). c PtME protein expression detected by western blotting with an anti-Flag antibody; beta-actin was used as internal reference protein. d Enzymatic activity of ME in transformed and wild type cells. **indicates a significant difference between wild type and engineered microalgae (P < 0.01)