From: Genetic basis for hyper production of hyaluronic acid in natural and engineered microorganisms
Microorganism | Genetic modification | Substrate concentration (g/L) | Production (g/L) | Yield YP/S (g/g) | Productivity (g/L/h) | Molecular weight (MDa) | Reference |
---|---|---|---|---|---|---|---|
Lactococcus lactis BCRC 12312 | Operon containing HA synthase (HasA) of S. equi subsp. zooepidemicus | Glucose: 0.1 | 0.08 | 0.8a | 0.0022b | NR | [62] |
Lactococcus lactis BCRC 12312 | Operon containing HA synthase (HasA) and UDP-GlcDH (HasB) of S. equi subsp. zooepidemicus | Glucose: 0.1 | 0.65 | 6.5a | 0.018b | NR | [62] |
Lactococcus lactis NZ9000 | Expression of HA synthase and UDP-glucose-6-dehydrogenase of Streptococcus zooepidemicus | Fed batch of glucose: 5 and fed batch of lactose: 20 | 0.12 | NR | NR | 0.879 | [65] |
0.59 | NR | NR | 0.569 | ||||
Lactococcus lactis NZ9000 (SJR2) | has operon of Streptococcus zooepidemicus (co-expressing HasA and HasB genes only) | Glucose: 15 | 0.107 (in static flask experiments) | 0.007a | 0.0178 | NR | [61] |
Glucose: 25 | 0.068 (in static flask experiments) | 0.002a | 0.0113 | NR | |||
Lactococcus lactis NZ9000 (SJR3) | has operon of Streptococcus zooepidemicus (co-expressing HasA, HasB, and HasC genes) | Glucose: 10 | 0.234 (in static flask experiments) | 0.023a | 0.039 | NR | |
Glucose: 25 | 0.154 (in static flask experiments) | 0.006a | 0.257 | NR | |||
Lactococcus lactis NZ9000 (SJR2) | has operon of Streptococcus zooepidemicus (co-expressing HasA and HasB genes only) | Glucose: 10 | 0.123 | 0.012 | 0.005b | NR | [63] |
Lactococcus lactis NZ9000 (SJR3) | has operon of Streptococcus zooepidemicus (co-expressing HasA, HasB, and HasC genes) | Glucose: 10 | 0.43 | 0.041 (YP/S) | 0.0179b | NR | [63] |
Lactococcus lactis NZ9000 (SJR6) | Three has operon genes (HasA, HasB and glmU) from S. zooepidemicus | Glucose: 10 | 0.595 | 0.09Â g/g (YP/S) | 0.0248b | NR | |
L. lactis NZ9000 | L. lactis carrying HasA- HasB gene in nisRK region | Glucose: 10 | 0.14 | 0.014a | 0.0117 | 4.3 | [77] |
VRJ2AB | |||||||
Lactococcus lactis NZ9000 VRJ3ABC | L. lactis carrying HasA- HasB- HasC gene in the nisRK region | Glucose: 10 | 0.68 | 0.068 | 0.0567 | 3.49 | [77] |
Enterococcus faecalis OGlRF | Transposon 916 insertional mutagenesis | THY broth | 0.002-0.69 | ND | ND | ND | [64] |
Was subcloned into a plasmid shuttle vector (pAT19 and pPD41) | |||||||
Corynebacterium glutamicum ATCC 13032 pJH174.1 | HasA expression | MEK700 with glucose: 40 | Between 0.3 and 0.4 | 0.017 | ~0.003b | >1.4 | [87] |
CGXII with glucose: 40 | ~1.0 | 0.023 | ~0,008b | <0.27 | |||
Corynebacterium glutamicum ATCC 13032 pJH181.3 | HasA and HasC coexpression | MEK700 with glucose: 40 | Between 0.3 and 0.4 | 0.016 | ~0.003b | >1.4 | |
CGXII with glucose: 40 | Between 1.0 and 1.3 | 0.025 | ~0.01b | <0.27 | |||
Corynebacterium glutamicum ATCC 13032 pJH182.1 | HasA and vgb (bacterial haemoglobin from Vitreoscilla sp.) coexpression | MEK700 with glucose: 40 | Between 0.1 and 0.2 | 0.075 | ~0.001b | >1.4 | |
CGXII with glucose: 40 | ~0.8 | 0.014 | ~0.007b | <0.27 | |||
Corynebacterium glutamicum ATCC 13032 pJH183.2 | HasA, HasC and HasB coexpression | MEK700 with glucose: 40 | Between 0.3 and 0.4 | 0.017 | ~0.003b | >0.67 | |
CGXII with glucose: 40 | ~1.3 | 0.027 | ~0.01b | <0.27 | |||
Corynebacterium glutamicum ATCC 13032 pJH195.2 | HasA and glmU (from Pseudomonas putida KT2440) coexpression | MEK700 with glucose: 40 | ~0.3 | 0.014 | ~0.003b | >1.4 | |
CGXII with glucose: 40 | Between 1.0 and 1.3 | 0.026 | ~0.01b | <0.27 | |||
Corynebacterium glutamicum ATCC 13032 pJH196.2 | HasA, HasC and glmU coexpression | MEK700 with glucose: 40 | Between 0.3 and 0.4 | 0.017 | ~0.003b | >1.4 | |
CGXII with glucose: 40 | ~1.2 | 0.027 | ~0.01b | <0.27 | |||
Corynebacterium glutamicum ATCC 13032 pJH197.1 | HasA, glmU (from Pseudomonas putida KT2440) and HasC coexpression | MEK700 with glucose: 40 | <0.3 | 0.013 | ~0.003b | >0.67 | |
CGXII with glucose: 40 | Between 1.0 and 1.3 | 0.025 | ~Â ~0.01b | <0.27 | |||
Agrobacterium sp ATCC31749 | Coexpression of HA synthase from Pasteurella multocida, and UDP-glucose dehydrogenase from Escherichia coli | Sucrose: ~42.8, Lactose: 3.4 | ~3.0 | 0.0645a | 0.5b | 1.56 | [47] |
Agrobacterium sp LTU261 | Coexpression of HA synthase from Pasteurella multocida, and UDP-glucose dehydrogenase from Escherichia coli | ~2.3 | 0.0495a | 0.38b | 2.17 | ||
Agrobacterium sp LTU265 | Coexpression of HA synthase from Pasteurella multocida, and UDP-glucose dehydrogenase from Escherichia coli | ~2.4 | 0.06a | 0.4b | 0.72 | ||
Escherichia coli JM109 | Co-expression of HA synthase from Pasteurella | Terrific Broth/feeding medium contained 0.50 glucose (after feeding: glucose: 50) (Bioreactor) | 2.0 | 0.027 | 0.0127 | NR | [48] |
3.8 (fed-batch fermentation process in a 1Â L | |||||||
Multocida and uridine diphosphate-glucose dehydrogenase from E. coli K5 strain | Bioreactor) | ||||||
Escherichia coli OP50 | CPS1 cDNA from C. neoformans | NR | NR | NR | ~4 ng/hr/µg protein | NR | [39] |
Escherichia coli x1448 | Transposon 916 insertional mutagenesis was subcloned into a plasmid shuttle vector (pPD41, pPD41Δ4, pPD41 Δ5 and pPD41 Δ6) | THY broth | 0.002–0.08 | NR | NR | NR | [64] |
Escherichia coli K12 MG1655 sseAB | Gene of sse HasA with identical protein sequence of seHAS from Streptococcus equisimilis | Glucose: 10 (fed batch) | 0.155 (24Â h) | 0.0155 (24Â h)a | 0.0065 (24Â h) | 0.38 (24Â h) | [69] |
0.196 (48Â h) | 0.0196 (48Â h)a | 0.0041 (48Â h) | 0.5Â M (48Â h) | ||||
0.202 (72Â h) | 0.0202 (72Â h)a | 0.0028 (72Â h) | 1.7 (72Â h) | ||||
Escherichia coli K12 MG1655 sseABC | Gene of sse HasA with identical protein sequence of seHAS from Streptococcus equisimilis | Glucose: 10 (fed batch) | 0.148 (48Â h) | 0.0148a | 0.0031 | 0.39 | [69] |
Escherichia coli C0 Top10/(pMBAD-sseABC, pHACM-blank) | Two plasmids and the chromosomal copies of wildtype rpoD and rpoS. | Glucose: 10 (fed batch) | 0.405 | 0.0405a | 0.008 | NR | [92] |
Escherichia coli C1 Top10/(pMBAD-sseABC) | Used as the control strain in library screening, harboring a single plasmid and the chromosomal copies of wild type rpoD and rpoS | Glucose: 10 (fed batch) | 0.510 | 0.0510a | 0.0106 | NR | [92] |
Escherichia coli D72 Top10/(pMBAD-sseABC, pHACM-rpoDM72) | Extra copy of mutant rpoD or rpoS in the pHACM plasmid | Glucose: 10 (fed batch) | 0.561 | 0.0561a | 0.0117 | NR | [92] |
Escherichia coli D2 Top10/(pMBAD-sseABC, pHACM-rpoDM2) | Extra copy of mutant rpoD or rpoS in the pHACM plasmid | Glucose: 10 (fed batch) | 0.548 | 0.0548a | 0.0114 | NR | [92] |
Escherichia coli S47 Top10/(pMBAD-sseABC, pHACM-rpoSM47) | Extra copy of mutant rpoD or rpoS in the pHACM plasmid | Glucose: 10 (fed batch) | 0.479 | 0.0479a | 0.00998 | NR | [92] |
Escherichia coli D0 Top10/(pMBAD-sseABC, pHACM-rpoD) | With an extra copy of unmutated rpoD on pHACM; | Glucose: 10 (fed batch) | 0.425 | 0.0425a | 0.009 | NR | [92] |
Escherichia coli S0 Top10/(pMBAD-sseABC, pHACM-rpoS) | With an extra copy of unmutated rpoS on pHACM; | Fed batch of glucose: 10 | 0.696 | 0.0696a | 0.015 | NR | [92] |
Streptomyces albulus pJHA4 | hasA gene from 29 Streptococcus zooepidemicus | Fed batch of glucose glucose: 50 | 6.2 | 0.062a | 0.886b | 2 | [70] |
Bacillus subtilis | Operon containing HA synthase (HasA) of Streptococcus, tuaD (hasB) | Fed batch rate of sucrose: 2 | NR | 1 | NR | 4.0 | [71] |
Bacillus subtilis | Operon containing HA synthase (HasA) of Streptococcus, tuaD (hasB) and Vitreoscilla hemoglobin (VHb) | Glucose: 10 | 1.8 | 0.18a | 0.6b | NR | [64] |
Bacillus subtilis | Operon containing HA synthase (HasA) of Streptococcus and tuaD controlled by inductive promoters | Glucose: 10 | 6.8 | 0.68a | 0.34b | 6.5 | [72] |