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Fig. 4 | Microbial Cell Factories

Fig. 4

From: Application of a delta-6 desaturase with α-linolenic acid preference on eicosapentaenoic acid production in Mortierella alpina

Fig. 4

The effect of different concentrations of PSO on each strain growth and principal fatty acid profiles at low temperature. DCW (a), TFA (b), AA level (c), EPA level (d), intracellular ALA level (e), the percentage of EPA (f) were determined in the control strain (WT M. alpina) and transformants (including Ma-MaFADS6-I-1, Ma-MaFADS6-I-2, Ma-MpFADS6-1, Ma-MpFADS6-2 and Ma-MpFADS6-3). Mycelia were cultured for 3 days at 28 °C and then at 12 °C for 8 days in Broth medium containing PSO-free, lower concentrations of PSO [0.025, 0.05 or 0.1 % (v/v) PSO] or higher concentration of PSO [0.2 % (v/v) PSO] as the exogenous substrate. All values are the means of three independent experiments and error bars represent standard deviations. TFA (g/L) = MTFA of 50 mg freeze-dried cells × MCDW of 50 mL cultures/50 mg × 20

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