Fig. 2
The effect of constant temperature and low temperature conditions on each strain growth and principal fatty acid profiles without any substrate. DCW (dry cell weight) (a), TFA (total fatty acids) (b), LA level (c), ALA level (d), AA level (e), EPA level (f), the percentage of EPA (g) and the amount of GLA and DGLA (h) were determined in the control strain (WT M. alpina) and transformants (including Ma-MaFADS6-I-1, Ma-MaFADS6-I-2, Ma-MpFADS6-1, Ma-MpFADS6-2 and Ma-MpFADS6-3). Mycelia were cultured under constant temperature (7 days at 28 °C) or low temperature (3 days at 28 °C and then at 12 °C for 8 days) in Broth medium. All values are the means of three independent experiments and error bars represent standard deviations. TFA (g/L) = MTFA of 50 mg freeze-dried cells × MCDW of 50 mL cultures/50 mg × 20