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Table 1 Conjugation results showing that the plasmid backbone has an effect on achieving successful editing

From: CRISPR/Cas9 mediated targeted mutagenesis of the fast growing cyanobacterium Synechococcus elongatus UTEX 2973

Construct Total number of exconjugants Percent of exconjugants edited and segregated on 1st patch
pVZ321 >250
pVZ321 + sSpcas9 0
pVZ321 + nblA sgRNA, ∆nblA editing template, sSpcas9 0 N/A
pVZ321 + nblA sgRNA, ∆nblA editing template, sSpcas9 (no promoter) 0 N/A
pVZ321 + nblA sgRNA, ∆nblA editing template, sSpcas9 (no RBS) 4 0/4 (0 %)
pCRISPomyces-2 + nblA sgRNA, ∆nblA editing template, sSpcas9 21 16/16 (100 %)
pCRISPomyces-2 + nblA sgRNA, ∆nblA repair template—sSpcas9 32 3/10 (30 %)