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Table 1 Overview of produced target molecules with advanced growth system

From: The fed-batch principle for the molecular biology lab: controlled nutrient diets in ready-made media improve production of recombinant proteins in Escherichia coli

Target group Specific target References
Enzymes Monomeric variants of Trypanosoma brucei triosephosphate isomerase(TIM) [21]
Drosophila melanogaster multifunctional enzyme type 2 (MFE2) [11]
A-domain of human protein disulphide isomerase (PDI) [11]
Lactobacillus alcohol dehydrogenase (ADH) [11]
Tryptophan synthase complex [52]
Formate dehydrogenase (FDH) from Candida boidinii [93]
Human NSUN4, 5-methylcytosine RNA methyltransferase [34]
SdrE protein—surface adhesin of Staphylococcus aureus [35]
Zmp1—zinc-dependent metalloprotease [36]
Human lipoxygenases hALOX15 [38]
Esterase, Est 22; Axe A and Axe B [44, 54]
Rhizopus oryzae lipase (ROL) [84]
Purine nucleoside phosphorylases (PNPs) [26, 103105]
Alkaline phosphatase (PhoA) and a phytase, AppA [58]
Surface lipoprotein SitA from Staphylococcus pseudointermedius [42]
Endopolygalacturonase from Aspergillus niger [32]
Chloramphenicol acetyltransferase I (CATI) [45]
Alkane monooxygenase (AlkB) [43]
Pseudomonas aeruginosa lipoxygenase (PA-LOX) [39]
Sortases: SrtC1-2b, Srt2-2b, SrtA [46]
Nitrilases [51]
Rat lactate dehydrogenase [53]
Zebrafish ALOX2 (lipoxygenase) [40]
Zinc‑dependent metalloprotease Zmp1 [101]
α-amylase AmyM from Geobacillus stearothermophilus [88]
Rhizopus oryzae lipase (ROL) in P. pastoris [84]
Antibodies, antibody fragments, other binding proteins Two single-domain antibodies (sdAbs) [68]
Four Fab fragments [24]
Anti-interleukin 1ß scFv [58]
Zinc transporter 8 autoantibodies (ZnT8A) [49]
Different nanobody variants [60]
10 scFv and 10 Fab fragments [70]
DNA binding protein LiaR [41]
DNA binding transcriptional regulator NadR (Neisseria adhesin A regulator) [100]
RNA-binding proteins: nucleolin EF-hand domain-containing protein D2 (EFHD2), splicing factor U2AF (U2AF653) serine/arginine-rich splicing factor 1 (SRSF1) [47]
Fully reduced active RNase inhibitor: contains leucine-rich repeat motifs (LRR) [22, 48, 98]
Disulfide bonded proteinsa Reteplase, a fragment of tissue plasminogen activator which contains only the kringle 2 and protease domains (nine disulfide bonds) [7]
Chimeric-truncated form of tissue-type plasminogen activator (t-PA) [33]
Human leukemia inhibitory factor (hLIF)—a cytokine (three intramolecular disulfide bonds) [50]
AppA, a phytase (four disulfide bonds) [58]
Human growth hormone hGH [58]
Human-derived glycosyltransferase GalNAcT2 (five disulfide bonds) [59]
Bioactive peptides Super-large non-ribosomal peptide synthetase valinomycin synthetase subunits Vlm1 (370 kDa) and Vlm2 (284 kDa) [72]
Nonribosomal peptide valinomycin [73, 75, 106]
Polyketide macrolide 6-deoxyerythronolide B (6dEB) in Bacillus subtilis [76]
Ribosomal peptide microviridin [71]
Others Plasmid DNA [7779]
Capripoxvirus proteins 64 and 95 [25]
Fusion protein: multiple-epitope antigen CTB-UE [69]
Saporin L3 from Saponaria officinalis in Pichia pastoris [81]
Human immunoglobulin receptors in P. pastoris (extracellularly) [83]
  1. aAntibodies and antibody fragments are mentioned in a separate section