Table 1 Overview of produced target molecules with advanced growth system

Enzymes

Monomeric variants of Trypanosoma brucei triosephosphate isomerase(TIM)

[21]

Drosophila melanogaster multifunctional enzyme type 2 (MFE2)

[11]

A-domain of human protein disulphide isomerase (PDI)

[11]

Lactobacillus alcohol dehydrogenase (ADH)

[11]

Tryptophan synthase complex

[52]

Formate dehydrogenase (FDH) from Candida boidinii

[93]

Human NSUN4, 5-methylcytosine RNA methyltransferase

[34]

SdrE protein—surface adhesin of Staphylococcus aureus

[35]

Zmp1—zinc-dependent metalloprotease

[36]

Human lipoxygenases hALOX15

[38]

Esterase, Est 22; Axe A and Axe B

[44, 54]

Rhizopus oryzae lipase (ROL)

[84]

Purine nucleoside phosphorylases (PNPs)

[26, 103–105]

Alkaline phosphatase (PhoA) and a phytase, AppA

[58]

Surface lipoprotein SitA from Staphylococcus pseudointermedius

[42]

Endopolygalacturonase from Aspergillus niger

[32]

Chloramphenicol acetyltransferase I (CATI)

[45]

Alkane monooxygenase (AlkB)

[43]

Pseudomonas aeruginosa lipoxygenase (PA-LOX)

[39]

Sortases: SrtC1-2b, Srt2-2b, SrtA

[46]

Nitrilases

[51]

Rat lactate dehydrogenase

[53]

Zebrafish ALOX2 (lipoxygenase)

[40]

Zinc‑dependent metalloprotease Zmp1

[101]

α-amylase AmyM from Geobacillus stearothermophilus

[88]

Rhizopus oryzae lipase (ROL) in P. pastoris

[84]

Antibodies, antibody fragments, other binding proteins

Two single-domain antibodies (sdAbs)

[68]

Four Fab fragments

[24]

Anti-interleukin 1ß scFv

[58]

Zinc transporter 8 autoantibodies (ZnT8A)

[49]

Different nanobody variants

[60]

10 scFv and 10 Fab fragments

[70]

DNA binding protein LiaR

[41]

DNA binding transcriptional regulator NadR (Neisseria adhesin A regulator)

[100]

RNA-binding proteins: nucleolin EF-hand domain-containing protein D2 (EFHD2), splicing factor U2AF (U2AF653) serine/arginine-rich splicing factor 1 (SRSF1)

[47]

Fully reduced active RNase inhibitor: contains leucine-rich repeat motifs (LRR)

[22, 48, 98]

Disulfide bonded proteins^a

Reteplase, a fragment of tissue plasminogen activator which contains only the kringle 2 and protease domains (nine disulfide bonds)

[7]

Chimeric-truncated form of tissue-type plasminogen activator (t-PA)

[33]

Human leukemia inhibitory factor (hLIF)—a cytokine (three intramolecular disulfide bonds)

[50]

AppA, a phytase (four disulfide bonds)

[58]

Human growth hormone hGH

[58]

Human-derived glycosyltransferase GalNAcT2 (five disulfide bonds)

[59]

Bioactive peptides

Super-large non-ribosomal peptide synthetase valinomycin synthetase subunits Vlm1 (370 kDa) and Vlm2 (284 kDa)

[72]

Nonribosomal peptide valinomycin

[73, 75, 106]

Polyketide macrolide 6-deoxyerythronolide B (6dEB) in Bacillus subtilis

[76]

Ribosomal peptide microviridin

[71]

Others

Plasmid DNA

[77–79]

Capripoxvirus proteins 64 and 95

[25]

Fusion protein: multiple-epitope antigen CTB-UE

[69]

Saporin L3 from Saponaria officinalis in Pichia pastoris

[81]

Human immunoglobulin receptors in P. pastoris (extracellularly)

[83]