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Fig. 5 | Microbial Cell Factories

Fig. 5

From: Enabling low cost biopharmaceuticals: high level interferon alpha-2b production in Trichoderma reesei

Fig. 5

Immunoblot showing the IFNα-2b production level in the M674 supernatant cultivated in Triab125 and Triab126. Bioreactor cultures were grown in 20 g/L yeast extract, 40 g/L cellulose, 80 g/L cellobiose, and 40 g/L sorbose, 5 g/L (NH4)2SO4, 5 g/L KH2PO4 at pH 4.5. The Triab126 cultivation was done with SBTI inhibitor feeding (0.4 mg/ml final concentration). The supernatants were diluted so that 0.025 µl could be loaded per well. The protein standards were loaded in the gel corresponding to 200 ng, 100 ng, and 50 ng of full length IFNα-2b. The full length IFNα-2b runs around 17 kDa and the carrier bound material runs at 75 kDa

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