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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Enabling low cost biopharmaceuticals: high level interferon alpha-2b production in Trichoderma reesei

Fig. 3

Immunoblot detecting IFNα-2b produced by 9-protease deletion strains. Transformants from pep8 (#2–3) and pep11 (#4–7) deletion strains were grown in duplicate using 24 well plates in TrMM with 8.6 g/L diammonium citrate, 5.4 g/L NaSO4, 100 mM PIPPS, 20 g/L spent grain extract, 40 g/L lactose at pH 4.5, shaking at 28 °C. M577 is the parental control (#1). a The supernatant from day 5 was diluted so that 0.5 µl could be loaded per well. The full length IFNα-2b runs around 17 kDa and the carrier bound material runs at 75 kDa. b The supernatant from day 7 was diluted so that 0.5 µl could be loaded per well

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