Fig. 2
Immunoblot detecting IFNα-2b produced by 9-protease deletion strains. Transformants from amp1(#1–3), slp7 (4), amp2 (#5–7), and sep1 (#9–12) deletion strains were grown in duplicate in 24 well plates in TrMM with 8.6 g/L diammonium citrate, 5.4 g/L NaSO4, 100 mM PIPPS, 20 g/L spent grain extract, 40 g/L lactose at pH 4.5, shaking at 28 °C. a The supernatant from day 6 was diluted so that 0.5 µl could be loaded per well. The M577 strain is the parental control (#8). The full length IFNα-2b runs around 17 kDa and the carrier bound material runs at 75 kDa. b Supernatant from day 7 was diluted so that 0.5 µl could be loaded per well. The M577 strain is the parental control (#8)