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Fig. 1 | Microbial Cell Factories

Fig. 1

From: Enabling low cost biopharmaceuticals: high level interferon alpha-2b production in Trichoderma reesei

Fig. 1

The design of the IFNα-2b expression construct. IFNα-2b expression is driven by the cbh1 promoter (p) and terminated with the cbh1 terminator (t). IFNα-2b is expressed as a cleavable fusion protein with CBHI. The NVISKR sequence between CBHI and IFNα-2b can be intracellularly cleaved by the KEX2 protease, which resides in the late golgi. The construct contains a hygromycin selection marker (hygR). The construct is targeted to the cbh1 locus via the cbh1p on the 5′ end and cbh1 3′ flank sequence

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