Cellulases and beyond: the first 70 years of the enzyme producer Trichoderma reesei

Table 2 Examples for the genetically engineering of T. reesei towards overproduction of a metabolite or interesting molecule

Substance produced	Substrate employed	T. reesei strain background	Genetic alteration	Maximum titer	Reference
Ethylene	Wheat straw, cellulose	T. reesei (viride) TL124	Ethylene forming enzyme of Pseudomonas syringae expressed under the cel7a promoter	1.06 µl h⁻¹ g⁻¹ dry weight	[169]
Ethylene	Wheat straw	QM9414	Ethylene forming enzyme of P. syringae expressed under the cel7a, pgk1 and A. nidulans gpdA promoters	4.012 nl h⁻¹ l⁻¹	[170]
N-acetyl-neuraminic acid	Chitin	QM9414	Expression of codon optimized N-acetyl glucosamine-2-epimerase from Anabaena sp. and N-acetyl neuramininc acid synthase from Campylobacter jejuni under the pki1 and xyn1 promoters	13 µg g⁻¹ mycelium	[171]
Xylitol	Barley straw	QM9414	Deletion of xylitol dehydrogenase, double deletion of xylitol dehydrogenase and l-arabinitol-4-dehydrogenase	13.2 g l⁻¹	[172]
Xylitol	Xylose and glucose	QM9414	Knockdown (antisense RNA) of xylulokinase; deletion of xylitol dehydrogenase, overexpression of d-xylose reductase	3.7 g l⁻¹	[173]
Erythritol	Wheat straw	RUT-C30, QM6a	Erythrose reductase expressed under the pki1 and bxl1 promoters	5 mg l⁻¹	[174]

ISSN: 1475-2859