Table 1 Bacterial strains and plasmids used in this study
Strains/plasmids | Relevant characteristics | Source or references |
|---|---|---|
Lactococcus lactis NZ9000 | MG1363 pepN::nisRK | [46] |
L. lactis NZ9000-pNZ401 | L. lactis NZ9000 harboring empty vector pNZ401 | This study |
L. lactis NZ9000-pNZ401-kiss1 | L. lactis NZ9000 harboring pNZ401-kiss1 | This study |
Escherichia coli DH5α | Cloning host | Takara |
E. coli DH5α-pNZ401 | E. coli DH5α harboring empty vector pNZ401 | Laboratory stock |
E. coli DH5α-pNZ401-kiss1 | E. coli DH5α harboring pNZ401- kiss1 | This study |
pNZ401 | Emr, inducible expression vector containing the nisA promoter, derivative of pNZ8048 | Laboratory stock |
pNZ401-kiss1 | pNZ401 carrying kiss1 gene | This study |