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Table 4 Composition of solubilization buffers used for CD spectroscopy

From: Recovery of bioactive protein from bacterial inclusion bodies using trifluoroethanol as solubilization agent

Buffer

Buffer composition

a

10 mM Tris–HCl, pH 8.5

b

8 M urea, 10 mM Tris–HCl, pH 8.5

c

6 M GdnHCl, 10 mM Tris–HCl, pH 8.5

d

0–50 % TFE, 10 mM Tris–HCl, pH 8.5

e

0–50 % TFE, 3 M urea, 10 mM Tris–HCl, pH 8.5

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Microbial Cell Factories

ISSN: 1475-2859

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