Skip to main content

Table 2 Purification of hGH from inclusion bodies using TFE as solubilization agent

From: Recovery of bioactive protein from bacterial inclusion bodies using trifluoroethanol as solubilization agent

Sample

Amount (mg)

Step yield (%)

Overall yield (%)

IBs

79.14

–

–

Solubilized protein

74.21

94

94

Refolded protein

64.83

87

82

DEAE purified protein

46.8

72

59

SEC purified protein

35.6

76

45

  1. Details of protein yield at each step of purification and step yield indicate the suitability of this current method for high recovery of protein from inclusion bodies of E. coli
Back to article page

Microbial Cell Factories

ISSN: 1475-2859

Contact us