Fig. 6From: Recovery of bioactive protein from bacterial inclusion bodies using trifluoroethanol as solubilization agentAnalysis of secondary and tertiary structure of hGH in solubilized state. a Far UV CD spectra of hGH in 8 M urea, 6 M GdnHCl and varying concentrations of TFE. b Far UV CD spectra of hGH in 8 M urea, 6 M GdnHCl and varying concentrations of TFE in presence of 3 M urea. c Size exclusion chromatogram of hGH incubated in Tris–HCl and 50 % TFE. d Fluorescence spectra of hGH incubated in different buffers. e Normalized fluorescence spectra of NATA incubated in different buffers. f Stern–Volmer plots for acrylamide quenching of hGH in presence of different buffersBack to article page