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Fig. 6 | Microbial Cell Factories

Fig. 6

From: Recovery of bioactive protein from bacterial inclusion bodies using trifluoroethanol as solubilization agent

Fig. 6

Analysis of secondary and tertiary structure of hGH in solubilized state. a Far UV CD spectra of hGH in 8 M urea, 6 M GdnHCl and varying concentrations of TFE. b Far UV CD spectra of hGH in 8 M urea, 6 M GdnHCl and varying concentrations of TFE in presence of 3 M urea. c Size exclusion chromatogram of hGH incubated in Tris–HCl and 50 % TFE. d Fluorescence spectra of hGH incubated in different buffers. e Normalized fluorescence spectra of NATA incubated in different buffers. f Stern–Volmer plots for acrylamide quenching of hGH in presence of different buffers

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