Skip to main content
Fig. 5 | Microbial Cell Factories

Fig. 5

From: Recovery of bioactive protein from bacterial inclusion bodies using trifluoroethanol as solubilization agent

Fig. 5

Expression of IBs of different proteins and their solubilization by TFE based buffer. a Expression of IBs of different proteins. Lane 1–9 is hGH, enolase, asparaginase, polyketide synthase, aldolase, ovalbumin, superoxide dismutase, ovine growth hormone and lysozyme IBs respectively, lane M LMW marker (97, 66, 45, 30, 20.1 and 14.5 kDa). b SDS-PAGE analysis of IB solubilization by TFE based buffer. Lane 1–9 are supernatant of hGH, enolase, asparaginase, polyketide synthase, aldolase, ovalbumin, superoxide dismutase, ovine growth hormone and lysozyme IBs solubilized in TFE based buffer respectively, lane M LMW marker (97, 66, 45, 30, 20.1 and 14.5 kDa)

Back to article page

Microbial Cell Factories

ISSN: 1475-2859

Contact us