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Fig. 4 | Microbial Cell Factories

Fig. 4

From: Recovery of bioactive protein from bacterial inclusion bodies using trifluoroethanol as solubilization agent

Fig. 4

Biophysical characterization of r-hGH. a Far UV CD spectra of r-hGH purified after solubilization in TFE based buffer along with that of hGH purified after solubilization in high pH buffer containing 2 M urea. b Near UV CD spectra of r-hGH purified after solubilization in TFE based buffer along with that of hGH purified after solubilization in high pH buffer containing 2 M urea. c Fluorescence spectra of r-hGH purified after solubilization in TFE based buffer along with that of hGH purified after solubilization in high pH buffer containing 2 M urea. d Nb2 cell proliferation assay of r-hGH purified after solubilization in TFE based buffer along with that of commercial hGH. Ovalbumin (ova) was used as negative control

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