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Fig. 2 | Microbial Cell Factories

Fig. 2

From: Recovery of bioactive protein from bacterial inclusion bodies using trifluoroethanol as solubilization agent

Fig. 2

Solubilization of hGH IBs in different concentrations of TFE in presence or absence of 3 M urea. a SDS-PAGE analysis of hGH IB solubilization. Lane 1–4 supernatant of hGH IBs solubilized in TFE concentrations ranging from 20 to 50 %, lane 5–8 supernatant of hGH IBs solubilized in TFE concentrations ranging from 10 to 40 % along with 3 M urea, lane 9 supernatant of hGH IBs solubilized in 3 M urea, lane 10 supernatant of hGH IBs solubilized in 8 M urea, lane M LMW marker (97, 66, 45, 30, 20.1, 14.4 kDa). b Turbidimetry of hGH IBs solubilized in different concentrations of TFE in absence and presence of 3 M urea at 380 nm

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Microbial Cell Factories

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