Gene | Primer | Sequences (5′–3′) | Usage |
---|---|---|---|
ME Plus 9 | ME Plus 9-5′ | CTGTCTCTTATACACATCTCAACCCTGA | Insertion mutant |
ME Plus 9-3′ | CTGTCTCTTATACACATCTCAACCATCA |  | |
pLys cm | cm-1-5′ | AACTGCAGTATCACTTATTCAGGCGTAGCA |  |
cm-1-3′ | CGAGCTCAATAAATCCTGGTGTCCCTGT |  | |
cm-SP1-5′ | ATTCACATTCTTGCCCGCCTGATG | Insertion location | |
cm-SP2-5′ | AGACGGTGAGCTGGTGATATGGGAT |  | |
cm-SP3-5′ | GAATACCACGACGATTTCCGGCAGT |  | |
cm-SP1-3′ | CGAAGAAGTTGTCCATATTGGCCACG |  | |
cm-SP2-3′ | AAACCCTTTAGGGAAATAGGCCAGGT |  | |
cm-SP3-3′ | TCACTCCAGAGCGATGAAAACGTTTC |  | |
cm-2-5′ | TATCACTTATTCAGGCGTAGCA | Gene disruption | |
cm-2-3′ | AATAAATCCTGGTGTCCCTGT |  | |
ZMO1122 | 1122-5′ | ATGAATAAACGCTATGATAACCGCAC |  |
1122-3′ | TTAGGCTCCCTTGATAATCCGC |  | |
1122-up-5′ | CGTTCAGCAGCCATATTTCTAGCCT |  | |
1122-up-cm-3′ | CAGGGACACCAGGATTTATTCGCAAGTAGGTGATTCTAAT |  | |
1122-down-cm-5′ | GTGCTACGCCTGAATAAGTGATACTTGTCTGATCGCGATGCCC |  | |
1122-down-3′ | AAGTCCCGCCCTTCTTTCCAAC |  | |
1122-F | TGAATAAACGCTATGATAAC | qPCR | |
1122-R | ATCTGTAATGTGACCAAG | Â | |
adhB | adhB-F | TACAACCTCGTCCTTCTT | Â |
adhB-R | CATAACCTTCTGCACTGA | Â | |
pdc | pdc-F | GGTATTAATTCTGCTGTT | Â |
pdc-R | CGAAGTCTGAATTGTTAT | Â | |
16S rDNA | 16S-F | TCAACTATAGACCAGTAAGT | Â |
16S-R | AGAACATAGAAGAGGTAAGT | Â |