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Table 4 List of primers used in the study (restrict sites underlined)

From: Engineered Zymomonas mobilis for salt tolerance using EZ-Tn5-based transposon insertion mutagenesis system

Gene

Primer

Sequences (5′–3′)

Usage

ME Plus 9

ME Plus 9-5′

CTGTCTCTTATACACATCTCAACCCTGA

Insertion mutant

ME Plus 9-3′

CTGTCTCTTATACACATCTCAACCATCA

 

pLys cm

cm-1-5′

AACTGCAGTATCACTTATTCAGGCGTAGCA

 

cm-1-3′

CGAGCTCAATAAATCCTGGTGTCCCTGT

 

cm-SP1-5′

ATTCACATTCTTGCCCGCCTGATG

Insertion location

cm-SP2-5′

AGACGGTGAGCTGGTGATATGGGAT

 

cm-SP3-5′

GAATACCACGACGATTTCCGGCAGT

 

cm-SP1-3′

CGAAGAAGTTGTCCATATTGGCCACG

 

cm-SP2-3′

AAACCCTTTAGGGAAATAGGCCAGGT

 

cm-SP3-3′

TCACTCCAGAGCGATGAAAACGTTTC

 

cm-2-5′

TATCACTTATTCAGGCGTAGCA

Gene disruption

cm-2-3′

AATAAATCCTGGTGTCCCTGT

 

ZMO1122

1122-5′

ATGAATAAACGCTATGATAACCGCAC

 

1122-3′

TTAGGCTCCCTTGATAATCCGC

 

1122-up-5′

CGTTCAGCAGCCATATTTCTAGCCT

 

1122-up-cm-3′

CAGGGACACCAGGATTTATTCGCAAGTAGGTGATTCTAAT

 

1122-down-cm-5′

GTGCTACGCCTGAATAAGTGATACTTGTCTGATCGCGATGCCC

 

1122-down-3′

AAGTCCCGCCCTTCTTTCCAAC

 

1122-F

TGAATAAACGCTATGATAAC

qPCR

1122-R

ATCTGTAATGTGACCAAG

 

adhB

adhB-F

TACAACCTCGTCCTTCTT

 

adhB-R

CATAACCTTCTGCACTGA

 

pdc

pdc-F

GGTATTAATTCTGCTGTT

 

pdc-R

CGAAGTCTGAATTGTTAT

 

16S rDNA

16S-F

TCAACTATAGACCAGTAAGT

 

16S-R

AGAACATAGAAGAGGTAAGT

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