Table 3 A comparison on the characteristics of N₂-fixing hydrogenotrophs of the order Methanococcales

Type strain

Mc-S-70

JJ

Nankai-3

SN1

Cell diameter (mm)

0.8–1.5

0.9–1.3

1.5–2.0

1.5

Substrates for methanogenesis

H₂ + CO₂, formate

H₂ + CO₂, formate

H₂ + CO₂, formate

H₂ + CO₂, formate

Autotrophy

+

+

+

+

Yeast extract stimulates growth

–

+

–

–

Selenium simulates growth

–

+

+

nd

Nitrogen source

NH₃, N₂, NO₃

NH₃, N₂, alanine

NH₃, N₂

NH₃, N₂, NO₃ ⁻

Sulfur source

S²⁻

S²⁻, S⁰

S²⁻, S⁰

S²⁻, S⁰, S₂O₃ ²⁻, SO₃ ²⁻, SO₄ ²⁻

Temperature range (°C)

55–83

<20–45

<20–55

17–70

Temperature optimum (°C)

75

35–40

46

60–65

pH range

6.0–8.5

6.5–8.0

5.5–7.5

4.9–9.8

pH optimum

6.7

6.8–7.2

7

5.1–7.5

NaCl range (%, w/v)

0.4–6.0

0.3–5

0.3–6

0.6–9.4

NaCl optimum (%, w/v)

2.4

0.6–2

1–2

2–4

Genome size (Mb)

1.82

1.66

1.57

1.69

GC content (mol  %)

33

33

32

34

Doubling time (hr)

0.5

2

1.3

~1

Source

Deep-sea black smoker chimney

Salt marsh sediments

Marine sediments

Coastal geothermally heated sea sediments

Reference

[78]

[3]

[77]

[130]