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Fig. 1 | Microbial Cell Factories

Fig. 1

From: Pichia pastoris MutS strains are prone to misincorporation of O-methyl-l-homoserine at methionine residues when methanol is used as the sole carbon source

Fig. 1

A typical RP-HPLC profile of a Nanobody and product-related variants. Purified Nanobody C produced in the wild type strain X-33 or in the MutS strain KM71H, was analysed on a reversed phase HPLC system coupled to an ESI-Q-TOF mass spectrometer. Several modifications could be identified such as oxidation (Δ+16 Da) and hexose (Δ+162 Da). An additional and unknown Δ−16 Da variant was present when Nanobody C was expressed in the MutS strain KM71H and not in the WT strain X-33. Similar observations were done with Nanobody A and B (Table 1)

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Microbial Cell Factories

ISSN: 1475-2859

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