Fig. 6

Binding study using isothermal titration calorimetry. \({\mathbf{C}}_{{\mathbf{4}}}^{{\mathbf{P}}} - {\mathbf{D}}^{{{\mathbf{PPxY}}}}\) titrant (a), \({\mathbf{C}}_{{\mathbf{4}}}^{{\mathbf{P}}} - {\mathbf{D}}^{{{\mathbf{PPxY}} ^* }}\) titrant (b). The top graphs show the heat response upon titration of \({\mathbf{C}}_{{\mathbf{4}}}^{{\mathbf{P}}} - {\mathbf{D}}^{{{\mathbf{WW}}}}\) with the proline-rich ligands. Bottom graphs represent the integrated peak areas per mole of ligand