Fig. 4

MALDI-TOF of \({\mathbf{C}}_{{\mathbf{4}}}^{{\mathbf{P}}} - {\mathbf{D}}^{{{\mathbf{PPxY}}}}\) treated with α(1-2,1-3,1-6) mannosidase and phosphatase. Untreated \({\mathbf{C}}_{{\mathbf{4}}}^{{\mathbf{P}}} - {\mathbf{D}}^{{{\mathbf{PPxY}}}}\) (a). The protein was digested with α-mannosidase (b), then with phosphatase (c), and finally digested again with α-mannosidase (d). Enzymes were thermally inactivated between consecutive digestions. See Table 2 for observed m/z values and theoretical masses of the indicated glycoforms