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Fig. 1 | Microbial Cell Factories

Fig. 1

From: Integrated omics approaches provide strategies for rapid erythromycin yield increase in Saccharopolyspora erythraea

Fig. 1

Key bioprocess parameters of cultivation of WT (a) and HP (b) strains in bioreactor scale. Erythromycin (ER) and glucose (Glc) concentrations, the packed mycelium volume (PMV), and relative expression of key erythromycin biosynthetic gene eryAI are plotted against total bioprocess time for a representative replicate of the bioprocess. The dashed lines denote the time points selected for transcriptomic and proteomic analysis. In the WT strain, erythromycin is produced primarily during the period of intense cell growth and rapid glucose consumption. In the HP strain, final erythromycin yield is much higher due to an increased rate of production during the rapid growth phase and a prolonged period of production in the late stages of the bioprocess. Expression profiles of eryAI and eryK erythromycin biosynthesis marker genes in both bioprocesses are shown in Additional file 3

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