Fig. 2

Metabolic pathways for biosynthesis of P(3HB) in recombinant R. eutropha strain from glucose and xylose as carbon sources used in this study. The overall metabolic pathway is shown together with the introduced metabolic pathways for the production of P(3HB). Xylulose 5-phosphate is generated by E. coli xylose isomerase (xylA) and xylulokinase (xylB). 3-Hydroxybutyryl-CoA is generated by R. eutropha β-ketothiolase (phaA) and acetoacetyl-CoA reductase (phaB). In R. eutropha strain, all the genes involved in PHA biosynthesis are in the chromosomal DNA except the xylAB gene encoding E. coli xylose isomerase and xylulokinase, which is additionally expressed by the introduction of pKM212-XylAB