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Table 3 plasmids used in this study

From: Characterization of genome-reduced Bacillus subtilis strains and their application for the production of guanosine and thymidine

plasmid

Genotype

Source

pU

pUC18, AmpR, containing cat-upp and two MCS regions

Lab stock

pCU

pUC18, AmpR, containing cat-upp and a MCS region

Lab stock

pU-pro1

pU, containing pro1 upstream, downstream flanks and an internal fragment in target region

This study

pU-pro2

pU, containing pro2 upstream, downstream flanks and an internal fragment in target region

This study

pCU-pro3

pCU, containing pro3 upstream and downstream flanks

This study

pU-pro4

pU, containing pro4 upstream, downstream flanks and an internal fragment in target region

This study

pU-pro5

pU, containing pro5 upstream, downstream flanks and an internal fragment in target region

This study

pU-pro6

pU, containing pro6 upstream, downstream flanks and an internal fragment in target region

This study

pU-PBSX

pU, containing upstream, downstream flanks of PBSX and an internal fragment in target region

This study

pU-skin

pU, containing upstream, downstream flanks of skin and an internal fragment in target region

This study

pU-spβ

pU, containing upstream, downstream flanks of spβ and an internal fragment in target region

This study

pU-spβ’

pU, containing upstream, downstream flanks of target region and an internal fragment in target region

This study

pU-yrkS-yraK

pU, containing upstream, downstream flanks of target and an internal fragment in target region

This study

pU-yisB-yitD

pU, containing upstream, downstream flanks of target and an internal fragment in target region

This study

pU-ycxB-sipU

pU, containing upstream, downstream flanks of target and an internal fragment in target region

This study

pU-pdp-rocR

pU, containing upstream, downstream flanks of target and an internal fragment in target region

This study

pCU-tdk

AmpR, CmR, containing tdk upstream and downstream flanks of target region

This study

pCU-purA

AmpR, CmR, containing purA upstream and downstream flanks

This study

pCU-bdhA-prs

AmpR, CmR, containing bdhA upstream and downstream flanks, P43- prs

This study

pHP13

CmR, EmR, B.subtils-E. coli shuttle plasmid

Lab stock

pHP13-ppg

pHP13 replication, CmR, EmR, P43-prs-purF-guaB

This study

pHP13-untd

pHP13 replication, CmR, EmR, P43-ushA-ndk-thyA-dut

This study

  1. AmpR, ampicillin resistance; CmR, chloramphenicol resistance; EmR, erythromycin resistance