plasmid | Genotype | Source |
---|---|---|
pU | pUC18, AmpR, containing cat-upp and two MCS regions | Lab stock |
pCU | pUC18, AmpR, containing cat-upp and a MCS region | Lab stock |
pU-pro1 | pU, containing pro1 upstream, downstream flanks and an internal fragment in target region | This study |
pU-pro2 | pU, containing pro2 upstream, downstream flanks and an internal fragment in target region | This study |
pCU-pro3 | pCU, containing pro3 upstream and downstream flanks | This study |
pU-pro4 | pU, containing pro4 upstream, downstream flanks and an internal fragment in target region | This study |
pU-pro5 | pU, containing pro5 upstream, downstream flanks and an internal fragment in target region | This study |
pU-pro6 | pU, containing pro6 upstream, downstream flanks and an internal fragment in target region | This study |
pU-PBSX | pU, containing upstream, downstream flanks of PBSX and an internal fragment in target region | This study |
pU-skin | pU, containing upstream, downstream flanks of skin and an internal fragment in target region | This study |
pU-spβ | pU, containing upstream, downstream flanks of spβ and an internal fragment in target region | This study |
pU-spβ’ | pU, containing upstream, downstream flanks of target region and an internal fragment in target region | This study |
pU-yrkS-yraK | pU, containing upstream, downstream flanks of target and an internal fragment in target region | This study |
pU-yisB-yitD | pU, containing upstream, downstream flanks of target and an internal fragment in target region | This study |
pU-ycxB-sipU | pU, containing upstream, downstream flanks of target and an internal fragment in target region | This study |
pU-pdp-rocR | pU, containing upstream, downstream flanks of target and an internal fragment in target region | This study |
pCU-tdk | AmpR, CmR, containing tdk upstream and downstream flanks of target region | This study |
pCU-purA | AmpR, CmR, containing purA upstream and downstream flanks | This study |
pCU-bdhA-prs | AmpR, CmR, containing bdhA upstream and downstream flanks, P43- prs | This study |
pHP13 | CmR, EmR, B.subtils-E. coli shuttle plasmid | Lab stock |
pHP13-ppg | pHP13 replication, CmR, EmR, P43-prs-purF-guaB | This study |
pHP13-untd | pHP13 replication, CmR, EmR, P43-ushA-ndk-thyA-dut | This study |