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Table 3 plasmids used in this study

From: Characterization of genome-reduced Bacillus subtilis strains and their application for the production of guanosine and thymidine

plasmid Genotype Source
pU pUC18, AmpR, containing cat-upp and two MCS regions Lab stock
pCU pUC18, AmpR, containing cat-upp and a MCS region Lab stock
pU-pro1 pU, containing pro1 upstream, downstream flanks and an internal fragment in target region This study
pU-pro2 pU, containing pro2 upstream, downstream flanks and an internal fragment in target region This study
pCU-pro3 pCU, containing pro3 upstream and downstream flanks This study
pU-pro4 pU, containing pro4 upstream, downstream flanks and an internal fragment in target region This study
pU-pro5 pU, containing pro5 upstream, downstream flanks and an internal fragment in target region This study
pU-pro6 pU, containing pro6 upstream, downstream flanks and an internal fragment in target region This study
pU-PBSX pU, containing upstream, downstream flanks of PBSX and an internal fragment in target region This study
pU-skin pU, containing upstream, downstream flanks of skin and an internal fragment in target region This study
pU-spβ pU, containing upstream, downstream flanks of spβ and an internal fragment in target region This study
pU-spβ’ pU, containing upstream, downstream flanks of target region and an internal fragment in target region This study
pU-yrkS-yraK pU, containing upstream, downstream flanks of target and an internal fragment in target region This study
pU-yisB-yitD pU, containing upstream, downstream flanks of target and an internal fragment in target region This study
pU-ycxB-sipU pU, containing upstream, downstream flanks of target and an internal fragment in target region This study
pU-pdp-rocR pU, containing upstream, downstream flanks of target and an internal fragment in target region This study
pCU-tdk AmpR, CmR, containing tdk upstream and downstream flanks of target region This study
pCU-purA AmpR, CmR, containing purA upstream and downstream flanks This study
pCU-bdhA-prs AmpR, CmR, containing bdhA upstream and downstream flanks, P43- prs This study
pHP13 CmR, EmR, B.subtils-E. coli shuttle plasmid Lab stock
pHP13-ppg pHP13 replication, CmR, EmR, P43-prs-purF-guaB This study
pHP13-untd pHP13 replication, CmR, EmR, P43-ushA-ndk-thyA-dut This study
  1. AmpR, ampicillin resistance; CmR, chloramphenicol resistance; EmR, erythromycin resistance