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Table 2 Strains used in this study

From: Characterization of genome-reduced Bacillus subtilis strains and their application for the production of guanosine and thymidine

Strain Genotype Source
E. coli DH5α Cloning host Invitrogen
BSF1 B.subtilis 168Δupp::neo [28]
BSK582 BSF1 Δpro1 Δpro2 Δpro3 Δpro5 Δpro6 Δspβ Δpps Δpks Δ(ycxB-sipU) Δ(yisB-yitD) Δ(pdp-rocR), 581.9 kb reduction of genome This study
BSK665 BSK582 ΔSpβ’ Δskin Δ(yybP-yyaJ), 665.2 kb reduction of genome This study
BSK756 BSK665 ΔPBSX Δ(ydeK-ydjC), 756.8 kb reduction of genome This study
BSK814 BSK756 Δpro4 Δ(lytH -yurT) Δ(sboA-ywhH), 814.4 kb reduction of genome This study
BSF1T1 BSF1 Δtdk This study
BSF1T2 BSF1 Δtdk ΔbdhA:: P43-prs This study
BSF1T3 BSF1 Δtdk ΔbdhA::P43-prs, containing pHP13-untd This study
BSK756T1 BSK756 Δtdk This study
BSK756T2 BSK756 Δtdk ΔbdhA:: P43-prs This study
BSK756T3 BSK756 Δtdk ΔbdhA::P43-prs, containing pHP13-untd This study
BSF1G1 BSF1 ΔpurA This study
BSF1G2 BSF1 ΔpurA, containing pHP13-ppg This study
BSK756G1 BSK756 ΔpurA This study
BSK756G2 BSK756 ΔpurA, containing pHP13-ppg This study
BSK814G1 BSK814 ΔpurA This study
BSK814G2 BSK814 ΔpurA, containing pHP13-ppg This study