Table 2 Strains used in this study
Strain | Genotype | Source |
|---|---|---|
E. coli DH5α | Cloning host | Invitrogen |
BSF1 | B.subtilis 168Δupp::neo | [28] |
BSK582 | BSF1 Δpro1 Δpro2 Δpro3 Δpro5 Δpro6 Δspβ Δpps Δpks Δ(ycxB-sipU) Δ(yisB-yitD) Δ(pdp-rocR), 581.9 kb reduction of genome | This study |
BSK665 | BSK582 ΔSpβ’ Δskin Δ(yybP-yyaJ), 665.2 kb reduction of genome | This study |
BSK756 | BSK665 ΔPBSX Δ(ydeK-ydjC), 756.8 kb reduction of genome | This study |
BSK814 | BSK756 Δpro4 Δ(lytH -yurT) Δ(sboA-ywhH), 814.4 kb reduction of genome | This study |
BSF1T1 | BSF1 Δtdk | This study |
BSF1T2 | BSF1 Δtdk ΔbdhA:: P43-prs | This study |
BSF1T3 | BSF1 Δtdk ΔbdhA::P43-prs, containing pHP13-untd | This study |
BSK756T1 | BSK756 Δtdk | This study |
BSK756T2 | BSK756 Δtdk ΔbdhA:: P43-prs | This study |
BSK756T3 | BSK756 Δtdk ΔbdhA::P43-prs, containing pHP13-untd | This study |
BSF1G1 | BSF1 ΔpurA | This study |
BSF1G2 | BSF1 ΔpurA, containing pHP13-ppg | This study |
BSK756G1 | BSK756 ΔpurA | This study |
BSK756G2 | BSK756 ΔpurA, containing pHP13-ppg | This study |
BSK814G1 | BSK814 ΔpurA | This study |
BSK814G2 | BSK814 ΔpurA, containing pHP13-ppg | This study |