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Table 1 Comparison of growth-related data of genome-reduced strains and the parental strain under aerobic conditions

From: Characterization of genome-reduced Bacillus subtilis strains and their application for the production of guanosine and thymidine

Strains

BSF1

BSK582

BSK665

BSK756

BSK814

Specific growth rate (h−1)a

0.54 ± 0.03

0.48 ± 0.03

0.43 ± 0.02

0.42 ± 0.02

0.41 ± 0.02

CDW (g L−1)b

1.62 ± 0.02

1.64 ± 0.01

1.80 ± 0.03

2.17 ± 0.02

2.20 ± 0.03

Specific glucose uptake rate (mmol g−1cdw h−1)

6.55 ± 0.32

6.20 ± 0.26

5.30 ± 0.50

5.05 ± 0.31

4. 87 ± 0.25

Glucose consumption (g L−1)

6.56 ± 0.12

6.19 ± 0.10

6.77 ± 0.03

6.44 ± 0.10

6.38 ± 0. 16

Biomass yield (g/g glucose)

0.26 ± 0.01

0.28 ± 0.01

0.29 ± 0.02

0.35 ± 0.01

0.36 ± 0.01

  1. Standard deviations were calculated from three biological replicates
  2. a Batch cultures were performed in 100 ml M9 medium with 1 % (w/v) glucose as described in method section
  3. b CDW is the final cell dry weight. At end of fermentation, cells were harvested and dried at 80 °C to a constant weight. Cell dry weight was determined by weighting the dried cells