Skip to main content

Advertisement

Table 1 Comparison of growth-related data of genome-reduced strains and the parental strain under aerobic conditions

From: Characterization of genome-reduced Bacillus subtilis strains and their application for the production of guanosine and thymidine

Strains BSF1 BSK582 BSK665 BSK756 BSK814
Specific growth rate (h−1)a 0.54 ± 0.03 0.48 ± 0.03 0.43 ± 0.02 0.42 ± 0.02 0.41 ± 0.02
CDW (g L−1)b 1.62 ± 0.02 1.64 ± 0.01 1.80 ± 0.03 2.17 ± 0.02 2.20 ± 0.03
Specific glucose uptake rate (mmol g−1cdw h−1) 6.55 ± 0.32 6.20 ± 0.26 5.30 ± 0.50 5.05 ± 0.31 4. 87 ± 0.25
Glucose consumption (g L−1) 6.56 ± 0.12 6.19 ± 0.10 6.77 ± 0.03 6.44 ± 0.10 6.38 ± 0. 16
Biomass yield (g/g glucose) 0.26 ± 0.01 0.28 ± 0.01 0.29 ± 0.02 0.35 ± 0.01 0.36 ± 0.01
  1. Standard deviations were calculated from three biological replicates
  2. a Batch cultures were performed in 100 ml M9 medium with 1 % (w/v) glucose as described in method section
  3. b CDW is the final cell dry weight. At end of fermentation, cells were harvested and dried at 80 °C to a constant weight. Cell dry weight was determined by weighting the dried cells