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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Characterization of genome-reduced Bacillus subtilis strains and their application for the production of guanosine and thymidine

Fig. 3

Engineering strategy for guanosine production. A deletion of the gene purA was combined with overexpression of the genes prs, purF, and guaB under the control of the strong P43 promoter. The gene abbreviations are as follows: purA encodes adenylosuccinate synthase; prs encodes ribose-phosphate pyrophosphokinase; purF encodes amidophosphoribosyltransferase; guaB encodes IMP dehydrogenase

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