A new regulatory mechanism controlling carotenogenesis in the fungus Mucor circinelloides as a target to generate β-carotene over-producing strains by genetic engineering

Table 4 β-carotene content in crgA-null derivatives of MU206 and MU218

Strain	Genetic background	crgA deletion	β-carotene(ug/g dry mass)
Strain	Genetic background	crgA deletion	Dark	Light
MU241	Wild-type	−	25.9 ± 2.6^a	667.3 ± 49.1^a
MU221	Wild-type	+	1481.8 ± 131.0^c	2003.1 ± 146.0^c
MU605	MU218	+	2621.0 ± 204.0^f	3559.0 ± 262.5^f
MU606	MU218	+	3001.9 ± 232.1^g	4009.2 ± 206.8^g
MU607	MU218	−	93.0 ± 16.6^a	1007.8 ± 40.0^ab
MU608	MU218	−	97.2 ± 9.9^a	1230.9 ± 54.1^a
MU609	MU206	+	1817.6 ± 139.6^d	2328.6 ± 152.9^cd
MU610	MU206	−	441.1 ± 16.1^b	1094.6 ± 47.4^ab
MU611	MU206	+	2089.8 ± 117.6^e	2879.0 ± 127.6^e
MU612	MU206	+	1931.2 ± 97.9^de	2591.6 ± 154.3^de
MU613	MU206	−	661.3 ± 32.0^b	1425.1 ± 51.4^b

^a–gValues are mean ± standard deviation of at least three independent experiments and values within a column with different superscript letters were significantly different (p < 0.05)

ISSN: 1475-2859