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Fig. 3 | Microbial Cell Factories

Fig. 3

From: A new regulatory mechanism controlling carotenogenesis in the fungus Mucor circinelloides as a target to generate β-carotene over-producing strains by genetic engineering

Fig. 3

Expression of carB (a) and carRP (b) genes in crgA-null mutants derived from MU206 and MU218. Copy number of the carB (a) and carRP (b) mRNAs in total RNA sample from the indicated strains was determined by Droplet Digital PCR. The carB and carRP mRNA copy numbers were normalised to the actin reference gene. All RNA samples were diluted 10 times and detected in triplicate. MU221, prototrophic crgA mutant derived from R7B; MU218 and MU206, derived from R7B, leuA −; MU605, MU606: crgA-null mutants derived from MU218; MU607, prototrophic strain derived from MU218; MU609 and MU611, crgA-null mutant derived from MU206; MU610, prototrophic strain derived from MU206

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Microbial Cell Factories

ISSN: 1475-2859

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