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Fig. 2 | Microbial Cell Factories

Fig. 2

From: A new regulatory mechanism controlling carotenogenesis in the fungus Mucor circinelloides as a target to generate β-carotene over-producing strains by genetic engineering

Fig. 2

Analysis of the crgA locus in knockout mutants. Genomic DNA from the indicated strains was amplified by PCR using primers cigC-F1 and crgA-R1 that amplified wild-type (4.0 kb) and mutant crgA alleles (6.5 kb) (Fig. 1), and the PCR products were analysed by agarose gel electrophoresis. MU605, MU606 and MU607 were derived from MU218, whereas MU609, MU610, MU611 and MU612 were derived from MU206. Marker, λ DNA digested with HindIII. Sizes of the DNA marker relevant fragments are indicated on the left side of the figure

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Microbial Cell Factories

ISSN: 1475-2859

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