Fig. 1From: A new regulatory mechanism controlling carotenogenesis in the fungus Mucor circinelloides as a target to generate β-carotene over-producing strains by genetic engineeringThe genomic structure of crgA wild-type locus and after disruption by homologous recombination. The replacement fragment used to disrupt crgA gene corresponded to a 6.5-kb PstI fragment derived from plasmid pVEN172. The positions of primers CigC F1/crgA R1 used to amplify the disrupted and wild-type crgA locus, and the expected sizes of the corresponding PCR products, are indicatedBack to article page