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Table 1 Transfer efficiency of cry6Aa-pHTMob02281 and pHTMob02281 in B. thuringiensis and B. cereus strains

From: Mob/oriT, a mobilizable site-specific recombination system for unmarked genetic manipulation in Bacillus thuringiensis and Bacillus cereus

Plasmid donors

Recipients

Efficiency

Plasmid donors

Recipients

Efficiency

 

BMB171Str

4.5 × 10−5

 

BMB171Str

3.4 × 10−5

 

YBT1520Str

8.2 × 10−6

 

YBT1520Str

9.3 × 10−6

cry6Aa-pHTMob02281

CT-43::Kan

3.0 × 10−5

pHTMob02281

CT-43::Kan

2.8 × 10−5

 

UW85R

2.3 × 10−5

 

UW85R

2.7 × 10−5

 

BC10987Str

5.3 × 10−6

 

BC10987Str

7.0 × 10−6

  1. Transfer efficiency = transconjugants/donor cells