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Fig. 7 | Microbial Cell Factories

Fig. 7

From: Mob/oriT, a mobilizable site-specific recombination system for unmarked genetic manipulation in Bacillus thuringiensis and Bacillus cereus

Fig. 7

Design and confirmation of zmaJ disruption in B. cereus UW85R. a Depiction of zmaJ disruption. b PCR detection with primers zmaJ-wS and zmaJ-wA. DNA templates were from: 1, UW85R; 2, zmaJ gene-disrupted strain BMB0263; 3, pBMB0263 (negative control); M, DNA Marker III. c LC–MS detection of a molecular weight corresponding to ZmA in the culture supernatants. The molecular weight corresponding to ZmA is indicated by a rectangular frame

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Microbial Cell Factories

ISSN: 1475-2859

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