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Fig. 5 | Microbial Cell Factories

Fig. 5

From: Mob/oriT, a mobilizable site-specific recombination system for unmarked genetic manipulation in Bacillus thuringiensis and Bacillus cereus

Fig. 5

Design and confirmation of amyE disruption in B. thuringiensis BMB171. a Depiction of amyE disruption. b PCR detection with primers amyE-wS and amyE-wA. DNA templates were from: 1 pBMB0260 (negative control); 2 BMB171; 3 amyE gene-disruption strain BMB0260; M Trans2 K Plus II DNA marker; c Sequence analysis of the deletion region of BMB0260

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Microbial Cell Factories

ISSN: 1475-2859

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