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Fig. 4 | Microbial Cell Factories

Fig. 4

From: Mob/oriT, a mobilizable site-specific recombination system for unmarked genetic manipulation in Bacillus thuringiensis and Bacillus cereus

Fig. 4

Map of the temperature-sensitive-mobilizable recombination vector pRec-mob1-Ts. Arrows indicate the direction of transcription or replication. The Mob-Spc cassette is shown in red, including the spectinomycin-resistance gene (spc), the mob02281 gene, and the two mini-oriT sites (24-bp). The orientations of the replication of E. coli (ori.Ec) and the ampicillin-resistance gene (amp) are shown in light green; the orientations of the replication of B. thuringiensis and the erythromycin-resistance gene (erm) are shown in purple. The purple box flanking ori.Bt (Ts) indicates that the replication orientation of the temperature-sensitive replication region of B. thuringiensis is not known

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