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Fig. 2 | Microbial Cell Factories

Fig. 2

From: Mob/oriT, a mobilizable site-specific recombination system for unmarked genetic manipulation in Bacillus thuringiensis and Bacillus cereus

Fig. 2

Effects of oriT deletions on Mob02281-mediated recombination. The 1–352 bp oriT1 and 1–370 bp oriT2 segments are numbered. The structures were shown in [37]. Arrows indicate the presence of inverted repeats (IRs). The nic site is indicated as a vertical arrowhead. The arrangements of the two oriT sequences with or without deletion mutations in each substrate plasmid are listed. Deleted oriT was cloned at the corresponding site into pBMBT10. pBMBmob1 was used as a helper plasmid containing the gene encoding a mobilization protein. The frequency of oriT-specific recombination for each substrate plasmid was estimated following the methods described in [37]

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