Microorganism-regulated mechanisms of temperature effects on the performance of anaerobic digestion

Lin, Qiang; He, Guihua; Rui, Junpeng; Fang, Xiaoyu; Tao, Yong; Li, Jiabao; Li, Xiangzhen

doi:10.1186/s12934-016-0491-x

Microbial Cell Factories

Table 4 The relative activities of specific populations in peak period and their correlations to the performances

From: Microorganism-regulated mechanisms of temperature effects on the performance of anaerobic digestion

	25 °C	35 °C	45 °C	50 °C	55 °C	Temperature	DCP	Acetic acid	Propionic acid	Butyric acid	NH₄ ⁺-N
*Firmicutes*
Unclassified Clostridiales	0.8 ± 0.2a	0.6 ± 0.1ab	0.5 ± 0b	0.6 ± 0ab	0.5 ± 0.1ab	−0.534*	−0.427	0.512	0.366	0.560*	−0.390
Unclassified Clostridiaceae	0.2 ± 0b	0.2 ± 0b	0.4 ± 0.2ab	0.3 ± 0b	1.1 ± 0.5a	0.494	0.092	−0.204	−0.449	−0.226	0.699**
Unclassified Lachnospiraceae	0.2 ± 0b	0.8 ± 0.2a	0.4 ± 0b	0.4 ± 0b	0.5 ± 0ab	0.152	0.068	−0.530*	−0.318	−0.554*	0.225
Lactobacillus	2.3 ± 0.2b	2.5 ± 1.8b	3.1 ± 1.5b	1.1 ± 0.1b	9.9 ± 1.4a	0.469	−0.169	−0.218	−0.472	−0.216	0.521*
Clostridium	0.3 ± 0.1c	0.4 ± 0.1bc	0.5 ± 0bc	0.6 ± 0ab	0.8 ± 0.1a	0.767**	0.311	−0.521*	−0.828**	−0.563*	0.931**
Sedimentibacter	0.4 ± 0b	0.1 ± 0c	0.1 ± 0c	0.1 ± 0c	0.9 ± 0.2a	0.313	−0.334	0.111	−0.319	0.07	0.274
Syntrophomonas	0.6 ± 0.2a	0.1 ± 0b	0.2 ± 0.1b	0.2 ± 0.1b	0.2 ± 0.1b	−0.549*	−0.45	0.824**	0.633*	0.758**	−0.507
Tepidimicrobium	0.7 ± 0.3	1.5 ± 0.9	0.9 ± 0.1	0.3 ± 0.1	1.1 ± 0.3	−0.082	−0.236	0.063	0.069	−0.037	−0.092
Pelotomaculum	0.6 ± 0.2b	0.5 ± 0.3b	0.8 ± 0.2b	9.1 ± 4a	0.6 ± 0.4b	0.304	0.582*	−0.244	−0.345	−0.222	0.347
*Bacteroidetes*
Ruminofilibacter	0.6 ± 0	1.2 ± 0.4	1.2 ± 0.2	2.9 ± 0.9	3 ± 2.4	0.435	0.247	−0.254	−0.424	−0.31	0.861**
OTU84	0.7 ± 0.1	0.8 ± 0.4	1 ± 0.2	1.2 ± 0.2	0.9 ± 0.2	0.298	0.516*	−0.145	−0.139	−0.222	0.355
Unclassified Bacteroidales	8.8 ± 0.4a	5.1 ± 2.1b	2.1 ± 0.1bc	0.4 ± 0c	0.4 ± 0c	−0.904**	−0.728**	0.865**	0.895**	0.832**	−0.986**
Prevotella	0.2 ± 0c	0.9 ± 0.4abc	1.6 ± 0.3a	0.7 ± 0.1bc	1.1 ± 0.1ab	0.463	0.41	−0.590*	−0.329	−0.558*	0.454
Bacteroides	1.4 ± 0.2b	5.1 ± 1.8a	3.4 ± 0.5ab	0.6 ± 0.1b	0.7 ± 0.1b	−0.316	−0.162	−0.193	0.21	−0.156	−0.305
Unclassified Porphyromonadaceae	0.3 ± 0	0.2 ± 0	0.3 ± 0	0.2 ± 0.1	0.3 ± 0.1	−0.049	−0.085	0.185	0.161	0.194	−0.047
*Euryarchaeota*
Methanosarcina	2.5 ± 0.4	2.8 ± 1	1.6 ± 1.1	6.4 ± 1.3	11.3 ± 7.1	0.441	0.163	−0.590*	−0.472	−0.216	0.686**
Methanoculleus	13.8 ± 0.7b	93.7 ± 39.8a	38 ± 13.6ab	35.1 ± 8.6ab	10.7 ± 1.8b	−0.164	0.059	−0.138	0.087	−0.197	−0.167
Methanobrevibacter	0.6 ± 0.1	0.4 ± 0.2	0.3 ± 0.1	0.3 ± 0.1	0.6 ± 0.2	−0.124	−0.445	0.263	0.064	0.283	−0.302
Methanobacterium	1.1 ± 0.6	0.6 ± 0.2	0.9 ± 0.3	1.3 ± 0.5	2.4 ± 1	0.379	0.054	−0.194	−0.389	−0.142	0.599*
*Thermotogae*
S1	0.7 ± 0b	1.4 ± 0.5b	3.1 ± 0.9a	3.7 ± 0.5a	0.7 ± 0.1b	0.332	0.746**	−0.479	−0.261	−0.44	0.409
*WWE1*
W22	7.6 ± 0.7a	1.9 ± 0.4b	1.3 ± 0.4b	0.8 ± 0.2b	0.9 ± 0.1b	−0.848**	−0.733**	0.960**	0.855**	0.957**	−0.895**
*Synergistetes*
Aminobacterium	0.7 ± 0.2b	1.7 ± 0.3ab	1.4 ± 0.2ab	4.7 ± 2.3a	1.8 ± 0.6ab	0.38	0.511	−0.388	−0.42	−0.374	0.536*

The data were based on 16S rDNA datasets
All data are presented as means ± standard deviations; values with different letters in a row mean significant difference at p < 0.05; bold italic fonts stand for phylum, while non-bold fonts stand for genus and 1 OTU
DCP daily CH4 production
** Significant at p < 0.01; * Significant at p < 0.05

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ISSN: 1475-2859

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