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Table 3 The relative activities of specific populations in stable period and their correlations to the performances

From: Microorganism-regulated mechanisms of temperature effects on the performance of anaerobic digestion

 

25 °C

35 °C

45 °C

50 °C

55 °C

Temperature

DCP

Acetic acid

Propionic acid

Butyric acid

NH4 +-N

Firmicutes

Unclassified Clostridiales

0.63 ± 0.12

0.61 ± 0.1

0.47 ± 0.02

0.5 ± 0.03

0.44 ± 0.02

−0.569*

−0.371

−0.390

0.523*

0.379

−0.943**

Unclassified Clostridiaceae

0.09 ± 0.04b

0.18 ± 0.05b

0.73 ± 0.29ab

1.41 ± 0.43a

1.24 ± 0.39a

0.736**

0.615*

0.472

−0.601*

−0.457

0.861**

Unclassified Lachnospiraceae

0.24 ± 0.05b

0.99 ± 0.11a

0.32 ± 0.03b

0.43 ± 0.04b

0.44 ± 0.06b

−0.058

−0.068

−0.003

−0.274

−0.468

−0.070

Clostridium

0.22 ± 0.05c

0.51 ± 0.14ab

0.44 ± 0.06bc

0.54 ± 0.08ab

0.75 ± 0.06a

0.712**

0.332

0.599*

−0.630*

−0.676**

0.878**

Unclassified Clostridia

1.08 ± 0.23a

0.82 ± 0.07ab

1.13 ± 0.09a

0.6 ± 0.06b

0.67 ± 0.06b

−0.489

−0.364

−0.085

0.662**

0.433

0.614*

Candidate order MBA08

0.76 ± 0.06c

1.44 ± 0.08a

1 ± 0.07b

0.78 ± 0.06c

0.93 ± 0.06bc

−0.120

−0.182

0.007

−0.225

−0.433

0.125

Unclassified Ruminococcaceae

1.24 ± 0.27a

0.39 ± 0.07b

0.54 ± 0.07b

0.64 ± 0.06b

0.61 ± 0.11b

−0.477

−0.406

−0.497

0.640*

0.772**

0.578*

Syntrophomonas

0.91 ± 0.3a

0.06 ± 0.03b

0.22 ± 0.07b

0.08 ± 0.02b

0.22 ± 0.07b

−0.581*

−0.569*

−0.436

0.727**

0.825**

0.305

Candidate order SHA-98

1.05 ± 0.07

1.53 ± 0.56

0.81 ± 0.11

0.68 ± 0.14

1.51 ± 0.25

−0.038

−0.398

−0.164

−0.205

−0.087

−0.047

Lactobacillus

1.3 ± 0.15b

2.33 ± 0.78b

7.91 ± 4.17ab

10.15 ± 2.01ab

16.51 ± 6.57a

0.693**

0.335

0.643**

−0.512

−0.398

0.945**

Sedimentibacter

0.18 ± 0.05b

0.31 ± 0.1b

0.15 ± 0.01b

0.15 ± 0.04b

0.61 ± 0.03a

0.415

−0.263

0.444

−0.303

−0.285

0.471

Tepidimicrobium

1.24 ± 0.32

0.76 ± 0.1

0.86 ± 0.1

1.15 ± 0.16

1.29 ± 0.29

0.110

−0.051

−0.099

0.044

0.210

0.191

Pelotomaculum

0.34 ± 0.34b

0.33 ± 0.33b

1.02 ± 0.01b

4.16 ± 1.08a

1.08 ± 0.9b

0.461

0.742**

0.106

−0.493

−0.320

0.533*

Bacteroidetes

Ruminofilibacter

1.19 ± 0.16

1.03 ± 0.19

1.45 ± 0.25

0.86 ± 0.33

5.3 ± 4.87

0.278

−0.098

0.308

−0.171

−0.119

0.596*

OTU84

7.62 ± 6.74

1.15 ± 0.37

1.1 ± 0.36

1.01 ± 0.35

0.43 ± 0.22

−0.443

−0.348

−0.384

0.449

0.525*

−0.828**

Unclassified Porphyromonadaceae

0.27 ± 0.11

0.51 ± 0.05

0.23 ± 0.05

0.31 ± 0.13

0.36 ± 0.08

−0.039

−0.139

−0.168

−0.244

−0.248

−0.065

Prevotella

0.59 ± 0.16

1.43 ± 0.45

1.42 ± 0.25

1 ± 0.19

0.99 ± 0.15

0.197

0.216

0.443

−0.289

−0.485

0.300

Unclassified Bacteroidales

6.59 ± 2.6a

2.57 ± 0.55ab

2.89 ± 0.73ab

0.81 ± 0.09b

0.45 ± 0.07b

−0.725**

−0.539*

−0.603*

0.708**

0.698**

−0.920**

Bacteroides

0.76 ± 0.29b

11.2 ± 4.37a

2.44 ± 0.82b

1.21 ± 0.21b

0.83 ± 0.2b

−0.241

−0.156

−0.256

−0.216

−0.268

−0.299

Euryarchaeota

Methanosarcina

2.66 ± 2.19b

6.05 ± 3.77b

2.69 ± 1.83b

9.4 ± 2.41b

18.54 ± 2.72a

0.628*

0.130

0.509

−0.551*

−0.391

0.747**

Methanobacterium

1.49 ± 0.79ab

0.11 ± 0.11b

2.9 ± 0.73a

1.44 ± 0.34ab

1.72 ± 0.42ab

0.262

0.254

0.420

0.081

0.043

0.355

Methanobrevibacter

1.79 ± 1.15

0.65 ± 0.34

0.34 ± 0.11

1.83 ± 0.4

1.15 ± 0.4

−0.080

0.013

−0.370

0.038

0.282

−0.147

Methanoculleus

17.34 ± 5.32b

106.57 ± 62.41a

3.98 ± 0.9b

12.69 ± 5.65b

7.88 ± 1.39b

−0.276

−0.221

−0.391

−0.186

−0.129

−0.402

Synergistetes

Aminobacterium

3.84 ± 0.27

3.63 ± 1.58

1 ± 0.06

2.42 ± 0.56

2.09 ± 1.3

−0.448

−0.387

−0.582*

0.135

0.334

−0.738**

Thermotogae

S1

1.08 ± 0.09

1.1 ± 0.25

0.76 ± 0.21

0.56 ± 0.08

0.71 ± 0.15

−0.586*

−0.604*

−0.395

0.458

0.341

−0.871**

WWE1

W22

3.9 ± 1.85a

0.58 ± 0.05b

2.13 ± 0.36ab

0.81 ± 0.2b

0.91 ± 0.43b

−0.486

−0.397

−0.372

0.616*

0.643**

−0.673**

  1. The data were based on 16S rDNA datasets
  2. All data are presented as means ± standard deviations; values with different letters in a row mean significant difference at p < 0.05; bold italic fonts stand for phylum, while non-bold fonts stand for genus and 1 OTU
  3. DCP daily CH4 production
  4. ** Significant at p < 0.01; * Significant at p < 0.05