Microorganism-regulated mechanisms of temperature effects on the performance of anaerobic digestion

Lin, Qiang; He, Guihua; Rui, Junpeng; Fang, Xiaoyu; Tao, Yong; Li, Jiabao; Li, Xiangzhen

doi:10.1186/s12934-016-0491-x

Microbial Cell Factories

Table 1 Relative abundances of main microbial populations in stable period and their correlations to the performances

From: Microorganism-regulated mechanisms of temperature effects on the performance of anaerobic digestion

	25 °C	35 °C	45 °C	50 °C	55 °C	Temperature	DCP	Acetic acid	Propionic acid	Butyric acid	NH₄ ⁺-N
*Firmicutes*	57.72 ± 9.67a	30.41 ± 1.51b	52.99 ± 1.17a	55.67 ± 0.57a	51.19 ± 2.74a	0.119	0.168	−0.007	0.168	0.354	0.145
Unclassified Clostridiales	12.54 ± 2.28b	7.08 ± 0.37c	18.9 ± 0.81a	16.68 ± 0.93ab	15.81 ± 2.56ab	0.524*	0.496	0.460	−0.198	−0.146	0.605*
Unclassified Clostridiaceae	10.72 ± 3.9a	2.87 ± 0.47b	2.99 ± 0.95b	1.92 ± 0.45b	2.78 ± 0.96b	−0.628*	−0.575*	−0.548*	0.688**	0.755**	−0.802**
Unclassified Lachnospiraceae	9.99 ± 1.61a	1.66 ± 0.07b	3.36 ± 0.38b	1.84 ± 0.12b	2.09 ± 0.12b	−0.722**	−0.608*	−0.549*	0.863**	0.941**	−0.753**
Clostridium	2.12 ± 0.58bc	1.47 ± 0.28c	4.73 ± 1.01a	3.5 ± 0.39ab	2.03 ± 0.42bc	0.284	0.549*	0.411	−0.097	−0.143	0.348
Unclassified Clostridia	0.74 ± 0.17c	0.61 ± 0.04c	2.12 ± 0.25b	4.77 ± 0.28a	5.1 ± 0.73a	0.873**	0.611*	0.516*	−0.716**	−0.493	0.900**
Candidate order MBA08	0.39 ± 0.01b	0.27 ± 0.02b	1.18 ± 0.13b	4.79 ± 0.44a	6.03 ± 0.79a	0.839**	0.488	0.460	−0.703**	−0.462	0.860**
Unclassified Ruminococcaceae	1.93 ± 0.28b	3.42 ± 0.27a	1.41 ± 0.25bc	0.94 ± 0.02c	0.83 ± 0.12c	−0.642**	−0.477	−0.434	0.271	0.116	−0.676**
Syntrophomonas	0.12 ± 0.03c	2.79 ± 1.07a	0.84 ± 0.24bc	2.41 ± 0.07ab	1.11 ± 0.21bc	0.223	0.378	−0.142	−0.669**	−0.579*	0.256
Candidate order SHA-98	0.25 ± 0.04c	0.24 ± 0.01c	1.36 ± 0.17b	3 ± 0.43a	2.39 ± 0.42a	0.836**	0.748**	0.482	−0.701**	−0.512	0.879**
Lactobacillus	1.65 ± 0.19ab	1.03 ± 0.17ab	1.98 ± 0.57a	1.57 ± 0.16ab	0.9 ± 0.14b	−0.162	0.206	−0.156	0.322	0.190	−0.234
Sedimentibacter	1.38 ± 0.45b	0.51 ± 0.06c	2.47 ± 0.12a	1.47 ± 0.17b	0.22 ± 0.04c	−0.110	0.350	0.061	0.298	0.161	−0.117
Tepidimicrobium	0.11 ± 0.01c	0.13 ± 0.02c	0.56 ± 0.06b	0.55 ± 0.03b	0.95 ± 0.16a	0.872**	0.417	0.730**	−0.619*	−0.522*	0.934**
Pelotomaculum	0.01 ± 0b	0.02 ± 0.01b	0.01 ± 0b	0.05 ± 0.01a	0.07 ± 0a	0.723**	0.283	0.405	−0.646**	−0.438	0.793**
*Bacteroidetes*	24.52 ± 7.78ab	29.16 ± 1.91a	23.01 ± 0.36ab	12.84 ± 2.18bc	4.13 ± 0.63c	−0.702**	−0.316	−0.453	0.506	0.300	−0.813**
Ruminofilibacter	0.59 ± 0.08c	6.17 ± 1.16b	11.42 ± 1a	8.12 ± 1.75b	0.5 ± 0.07c	0.215	0.667**	0.329	−0.218	−0.474	0.220
OTU84	0.44 ± 0.06c	4.95 ± 0.93b	9.42 ± 0.95a	6.6 ± 1.66ab	0.3 ± 0.07c	0.210	0.655**	0.322	−0.209	−0.461	0.217
Unclassified Porphyromonadaceae	2.68 ± 1.31b	3.19 ± 0.56ab	5 ± 0.44a	1.73 ± 0.35b	1.08 ± 0.2b	−0.280	−0.005	0.186	0.364	0.051	−0.338
Prevotella	10.13 ± 0.91a	0.93 ± 0.14b	0.2 ± 0.04b	0.25 ± 0.02b	0.22 ± 0.04b	−0.816**	−0.696**	−0.708**	0.877**	0.980**	−0.819**
Unclassified Bacteroidales	1.07 ± 0.55b	4.97 ± 0.41a	0.38 ± 0.02b	0.39 ± 0.04b	0.48 ± 0.02b	−0.431	−0.366	−0.280	0.064	−0.130	−0.448
Bacteroides	5.87 ± 4.1	0.21 ± 0.07	0.07 ± 0.02	0.09 ± 0.02	0.1 ± 0.02	−0.531*	−0.458	−0.425	0.606*	0.664**	−0.793**
*Euryarchaeota*	1.7 ± 0.66ab	0.5 ± 0.06b	3.08 ± 0.78a	2.64 ± 0.54a	2.26 ± 0.56ab	0.419	0.456	0.293	−0.150	−0.150	0.571*
Methanosarcina	1.31 ± 0.61ab	0.05 ± 0.02b	2.16 ± 0.85a	1.33 ± 0.36ab	0.5 ± 0.17ab	0.019	0.254	−0.040	0.180	0.115	0.028
Methanobacterium	0.03 ± 0.02c	0.02 ± 0.01c	0.02 ± 0c	0.49 ± 0.12b	0.85 ± 0.22a	0.714**	0.287	0.419	−0.594*	−0.366	0.791**
Methanobrevibacter	0.04 ± 0.01b	0.12 ± 0.04b	0.68 ± 0.13a	0.17 ± 0.05b	0.1 ± 0.02b	0.233	0.423	0.549*	−0.036	−0.283	0.262
Methanoculleus	0.1 ± 0.02b	0.05 ± 0.03b	0.07 ± 0.02b	0.32 ± 0.04a	0.39 ± 0.08a	0.706**	0.379	0.357	−0.566*	−0.323	0.754**
*Synergistetes*	0.42 ± 0.09c	1.22 ± 0.26a	0.92 ± 0.1ab	0.62 ± 0.06bc	0.42 ± 0.06c	−0.123	0.119	0.180	−0.030	−0.388	−0.154
Aminobacterium	0.15 ± 0.02b	0.77 ± 0.14a	0.2 ± 0b	0.19 ± 0.03b	0.14 ± 0.04b	−0.289	−0.177	−0.126	−0.023	−0.283	−0.314
*Thermotogae*	0.36 ± 0.04c	0.25 ± 0.02c	0.89 ± 0.15c	11.95 ± 2.11b	24.14 ± 0.58a	0.788**	0.262	0.493	−0.642**	−0.406	0.797**
S1	0.32 ± 0.05c	0.25 ± 0.02c	0.88 ± 0.15c	11.93 ± 2.11b	24.1 ± 0.58a	0.789**	0.262	0.494	−0.643**	−0.407	0.798**
*WWE1*	1.34 ± 0.7c	14.21 ± 1.97a	7.68 ± 1.3b	0.56 ± 0.16c	0.41 ± 0.11c	−0.295	−0.142	0.068	0.066	−0.285	−0.307
W22	1.34 ± 0.7c	14.21 ± 1.97a	7.68 ± 1.3b	0.56 ± 0.16c	0.41 ± 0.11c	−0.295	−0.142	0.068	0.066	−0.285	−0.307

The data (average relative abundance in genus level >0.1 %) were shown,except for Pelotomaculum
The data were based on 16S rDNA datasets
All data are presented as means ± standard deviations; values with different letters in a row mean significant difference at p < 0.05; bold italic fonts stand for phylum, while non-bold fonts stand for genus and 1 OTU
DCP daily CH₄ production
** Significant at p < 0.01; * Significant at p < 0.05

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