From: Increasing the production of (R)-3-hydroxybutyrate in recombinant Escherichia coli by improved cofactor supply
Cofactor
Vmax (μmol mg−1 min−1)
KM (μM)
kcat (s−1)
kcat/KM (s−1 μM−1)
NADPH
1.6 ± 0.2
70 ± 6
780 ± 100
11.1 ± 0.3
NADH
0.20 ± 0.02
24 ± 6
100 ± 7
4.2 ± 0.2