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Table 1 Kinetic parameters of the acetoacetyl-CoA reductase from H. boliviensis

From: Increasing the production of (R)-3-hydroxybutyrate in recombinant Escherichia coli by improved cofactor supply

Cofactor 

Vmax (μmol mg−1 min−1)

KM (μM)

kcat (s−1)

kcat/KM (s−1 μM−1)

NADPH

1.6 ± 0.2

70 ± 6

780 ± 100

11.1 ± 0.3

NADH

0.20 ± 0.02

24 ± 6

100 ± 7

4.2 ± 0.2

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Microbial Cell Factories

ISSN: 1475-2859

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